“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 ◽  
Vol 112 (2) ◽  
pp. 195-203 ◽  
Author(s):  
W.Neal Burnette
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Western Blotting ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Dodecyl Sulfate ◽  
Electrophoretic Transfer

scholarly journals Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.

1978 ◽  
Vol 79 (1) ◽  
pp. 281-285 ◽  
Author(s):  
W S Adair ◽  
D Jurivich ◽  
U W Goodenough
Keyword(s):  
Staphylococcus Aureus ◽  
Sodium Dodecyl Sulfate ◽  
Cell Membrane ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Dodecyl Sulfate ◽  
A Cell ◽  
Antigen Antibody

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

Efficacy and compatibility with mass spectrometry of methods for elution of proteins from sodium dodecyl sulfate–polyacrylamide gels and polyvinyldifluoride membranes

2004 ◽  
Vol 330 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Charlotte Sværke Jørgensen ◽  
Mitchell Jagd ◽  
Birgitte Kjær Sørensen ◽  
Jim McGuire ◽  
Vibeke Barkholt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Dodecyl Sulfate

Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate–polyacrylamide gels

2012 ◽  
Vol 423 (2) ◽  
pp. 253-260 ◽  
Author(s):  
Takeshi Yoshimura ◽  
Gen Yamada ◽  
Mai Narumi ◽  
Takako Koike ◽  
Akihiro Ishii ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Tissue Samples ◽  
Dodecyl Sulfate
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