Isolation and characterization of uridine diphosphate fructose from tubers of Jerusalem artichoke (Helianthus tuberosus L.)

1967 ◽  
Vol 119 ◽  
pp. 240-252 ◽  
Author(s):  
Y. Umemura ◽  
M. Nakamura ◽  
S. Funahashi
Keyword(s):  
Jerusalem Artichoke ◽  
Helianthus Tuberosus ◽  
Uridine Diphosphate ◽  
Isolation And Characterization

scholarly journals Characterization of Fructan Metabolism During Jerusalem Artichoke (Helianthus tuberosus L.) Germination

2018 ◽  
Vol 9 ◽  
Author(s):  
Jiao Jiao ◽  
Ji Wang ◽  
Mengjia Zhou ◽  
Xuyang Ren ◽  
Wenyue Zhan ◽  
...  
Keyword(s):  
Jerusalem Artichoke ◽  
Helianthus Tuberosus ◽  
Fructan Metabolism

scholarly journals Isolation and characterization of the monkey UGT2B30 gene that encodes a uridine diphosphate-glucuronosyltransferase enzyme active on mineralocorticoid, glucocorticoid, androgen and oestrogen hormones

2002 ◽  
Vol 365 (1) ◽  
pp. 213-222 ◽  
Author(s):  
Caroline GIRARD ◽  
Olivier BARBIER ◽  
David TURGEON ◽  
Alain BÉLANGER
Keyword(s):  
Cynomolgus Monkey ◽  
Genomic Organization ◽  
Human Kidney ◽  
Relative Activity ◽  
Pcr Analysis ◽  
Uridine Diphosphate ◽  
Lipophilic Compounds ◽  
Isolation And Characterization ◽  
Hormonal Steroids

The present study reports the genomic organization and the characterization of a novel cynomolgus monkey UDP-glucuronosyltransferase (UGT) enzyme, UGT2B30. UGT enzymes are microsomal proteins that catalyse the transfer of the glucuronosyl group from UDP-glucuronic acid (UDPGA) to a wide variety of lipophilic compounds, namely hormonal steroids. The 15kb UGT2B30 gene amplified by PCR showed a genomic organization similar to those encoding UGT2B human enzymes. The cDNA encoding UGT2B30 was isolated from a cynomolgus monkey prostate cDNA library, and the deduced amino acid sequence showed an identity of 94% with UGT2B19, a monkey isoform previously characterized. Stable expression of UGT2B30 protein in human kidney 293 (HK293) cells was assessed by Western-blot analysis and its conjugating activity was screened using 39 potential substrates. The UGT2B30 enzyme is active on many compounds of different classes, including testosterone, dihydrotestosterone, 5α-androstane-3α,17β-diol, androsterone, oestradiol, tetrahydroaldosterone and tetrahydrocortisone, with glucuronidation efficiencies (Vmax/Km ratios) ranging from 0.6 to 8.8μl·min−1·mg of protein−1. Reverse-transcriptase-PCR analysis revealed that the UGT2B30 transcript is expressed in several tissues, including prostate, testis, mammary gland, kidney, adrenals and intestine. The relative activity of UGT2B30 in comparison with other simian UGT2B isoforms, as well as its large variety of substrates, strongly suggest that this enzyme is essential to inactivation of several steroids.

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