De Novo synthesis of Escherichia coli glycogen is due to primer associated with glycogen synthase and activation by branching enzyme

1978 ◽  
Vol 190 (2) ◽  
pp. 385-397 ◽  
Author(s):  
Kichitaro Kawaguchi ◽  
Jeffrey Fox ◽  
Eric Holmes ◽  
Charles Boyer ◽  
Jack Preiss
Keyword(s):  
Escherichia Coli ◽  
De Novo ◽  
Glycogen Synthase ◽  
Branching Enzyme ◽  
De Novo Synthesis

De novo Synthesis and Assembly of rRNA into Ribosomal Subunits during Cold Acclimation in Escherichia coli

2016 ◽  
Vol 428 (8) ◽  
pp. 1558-1573 ◽  
Author(s):  
Lolita Piersimoni ◽  
Mara Giangrossi ◽  
Paolo Marchi ◽  
Anna Brandi ◽  
Claudio O. Gualerzi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cold Acclimation ◽  
De Novo ◽  
De Novo Synthesis ◽  
Ribosomal Subunits

scholarly journals Modular Optimization of Heterologous Pathways for De Novo Synthesis of (2S)-Naringenin in Escherichia coli

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e101492 ◽  
Author(s):  
Junjun Wu ◽  
Tiantian Zhou ◽  
Guocheng Du ◽  
Jingwen Zhou ◽  
Jian Chen
Keyword(s):  
Escherichia Coli ◽  
De Novo ◽  
De Novo Synthesis

scholarly journals Gene Products Required for De Novo Synthesis of Polysialic Acid in Escherichia coli K1

2006 ◽  
Vol 188 (5) ◽  
pp. 1786-1797 ◽  
Author(s):  
Ekaterina N. Andreishcheva ◽  
Willie F. Vann
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
De Novo ◽  
Polysialic Acid ◽  
Neonatal Meningitis ◽  
Gene Products ◽  
De Novo Synthesis ◽  
E Coli ◽  
Tract Infections

ABSTRACT Escherichia coli K1 is responsible for 80% of E. coli neonatal meningitis and is a common pathogen in urinary tract infections. Bacteria of this serotype are encapsulated with the α(2-8)-polysialic acid NeuNAc(α2-8), common to several bacterial pathogens. The gene cluster encoding the pathway for synthesis of this polymer is organized into three regions: (i) kpsSCUDEF, (ii) neuDBACES, and (iii) kpsMT. The K1 polysialyltransferase, NeuS, cannot synthesize polysialic acid de novo without other products of the gene cluster. Membranes isolated from strains having the entire K1 gene cluster can synthesize polysialic acid de novo. We designed a series of plasmid constructs containing fragments of regions 1 and 2 in two compatible vectors to determine the minimum number of gene products required for de novo synthesis of the polysialic acid from CMP-NeuNAc in K1 E. coli. We measured the ability of the various combinations of region 1 and 2 fragments to restore polysialyltransferase activity in vitro in the absence of exogenously added polysaccharide acceptor. The products of region 2 genes neuDBACES alone were not sufficient to support de novo synthesis of polysialic acid in vitro. Only membrane fractions harboring NeuES and KpsCS could form sialic polymer in the absence of exogenous acceptor at the concentrations formed by wild-type E. coli K1 membranes. Membrane fractions harboring NeuES and KpsC together could form small quantities of the sialic polymer de novo.

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