Isolation and characterization of a homogeneous isoenzyme of wheat germ acid phosphatase

1991 ◽  
Vol 288 (2) ◽  
pp. 621-633 ◽  
Author(s):  
Parvin P. Waymack ◽  
Robert L. Van Etten
Keyword(s):  
Acid Phosphatase ◽  
Wheat Germ ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of regulatory mutants from Schizosaccharomyces pombe involved in thiamine-regulated gene expression.

Genetics ◽  
1992 ◽  
Vol 130 (3) ◽  
pp. 445-449
Author(s):  
A M Schweingruber ◽  
H Fankhauser ◽  
J Dlugonski ◽  
C Steinmann-Loss ◽  
M E Schweingruber
Keyword(s):  
Acid Phosphatase ◽  
Schizosaccharomyces Pombe ◽  
Mrna Levels ◽  
Wild Type ◽  
Transcription Control ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Thiamine Transport ◽  
Regulated Gene Expression

Abstract Mutants from Schizosaccharomyces pombe deficient in the regulation of thiamine-repressible acid phosphatase have been isolated. Mutants expressing derepressed levels of the enzyme in the presence and absence of thiamine map in three genes, tnr1, tnr2 and tnr3. mRNA levels of the pho4 gene (coding for thiamine repressible acid phosphatase) and another thiamine-regulatable gene, thi3 (coding for a thiamine biosynthetic enzyme and corresponding to nmt1) are constitutively synthesized in the mutants. The mutants also exhibit constitutive thiamine transport which is thiamine repressible in wild type. The tnr3 mutants reveal a 10-20-fold higher intracellular thiamine level than tnr1 and tnr2 mutants and wild type. Mutants expressing repressed levels of thiamine-repressible acid phosphatase map in gene thi1. No or little amounts of pho4- and nmt1-specific mRNA can be detected. These mutants are impaired in thiamine uptake and are thiamine auxotrophic due to the inability to synthesize the thiazole moiety of the thiamine molecule. All tested tnr and thi1 alleles are recessive, and thi1 mutations are epistatic over tnr mutations. We assume that the thi1 and tnr genes are involved in thiamine-mediated transcription control.

scholarly journals Isolation and Characterization of Acid Phosphatase Mutants in Saccharomyces cerevisiae

1973 ◽  
Vol 113 (2) ◽  
pp. 727-738 ◽  
Author(s):  
Akio Toh-e ◽  
Yoshinami Ueda ◽  
Sei-Ichiro Kakimoto ◽  
Yasuji Oshima
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acid Phosphatase ◽  
Isolation And Characterization
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