Characterization of a resolved oxygen-evolving Photosystem II preparation from spinach thylakoids

1983 ◽  
Vol 724 (2) ◽  
pp. 201-211 ◽  
Author(s):  
Eric Lam ◽  
Barbara Baltimore ◽  
William Ortiz ◽  
Susan Chollar ◽  
Anastasios Melis ◽  
...  

Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.



1988 ◽  
Vol 27 (8) ◽  
pp. 1309-1311 ◽  
Author(s):  
Robert J. Kulawiec ◽  
Robert H. Crabtree ◽  
Gary W. Brudvig ◽  
Gayle K. Schulte


2010 ◽  
Vol 1797 (2) ◽  
pp. 160-166 ◽  
Author(s):  
Ryo Nagao ◽  
Tatsuya Tomo ◽  
Eri Noguchi ◽  
Saori Nakajima ◽  
Takehiro Suzuki ◽  
...  


2015 ◽  
Vol 44 (5) ◽  
pp. 2132-2137 ◽  
Author(s):  
Rebecca O. Fuller ◽  
George A. Koutsantonis ◽  
Ivan Lozić ◽  
Mark I. Ogden ◽  
Brian W. Skelton

An investigation inspired by the structure of the oxygen-evolving complex of photosystem II has resulted in the isolation and structural characterization of the first examples of Mn/Ca clusters supported by a calixarene ligand.





2004 ◽  
Vol 45 (9) ◽  
pp. 1168-1175 ◽  
Author(s):  
Takehiro Suzuki ◽  
Osamu Tada ◽  
Miki Makimura ◽  
Akihiko Tohri ◽  
Hisataka Ohta ◽  
...  


1992 ◽  
Vol 1100 (3) ◽  
pp. 251-258 ◽  
Author(s):  
Fredrik Nilsson ◽  
Kleoniki Gounaris ◽  
Stenbjörn Styring ◽  
Bertil Andersson


1989 ◽  
Vol 13 (5) ◽  
pp. 573-582 ◽  
Author(s):  
Richard Wales ◽  
Barbara J. Newman ◽  
Stephanie A. Rose ◽  
Darryl Pappin ◽  
John C. Gray


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