The effect of mono- and divalent salts on the rise and decay kinetics of EPR signal II in Photosystem II preparations from spinach

1985 ◽  
Vol 808 (1) ◽  
pp. 132-139 ◽  
Author(s):  
Michael Boska ◽  
Neil V. Blough ◽  
Kenneth Sauer
Keyword(s):  
Photosystem Ii ◽  
Decay Kinetics ◽  
Epr Signal ◽  
Epr Signal Ii ◽  
Divalent Salts ◽  
Kinetics Of

Trichloroacetate Affects the Redox Active Tyrosine 160 of the D2 Polypeptide of the Photosystem II Core

1997 ◽  
Vol 52 (11-12) ◽  
pp. 782-788 ◽  
Author(s):  
R. Li ◽  
N. Lin ◽  
C. Xu ◽  
Y. Shen ◽  
Keyword(s):  
Photosystem Ii ◽  
Redox Property ◽  
Obvious Effect ◽  
Redox Active ◽  
Treated Sample ◽  
Epr Signal ◽  
Epr Signal Ii ◽  
Small Hydrophobic

Abstract Trichloroacetate (TCA) affects the redox property of YD (tyrosine-160 on D2 polypeptide) after the removal of 33 kDa extrinsic polypeptide from the photosystem II (PSII) core. However, TCA has no obvious effect on the redox property of YZ , confirming that the environments around YZ and YD are quite different. The conclusion on the effects on YD is based primarily on the observation that Y D+ is not detected as the EPR signal IIslow when 33 kDa polypeptide is released by TCA -treatment. Dialysis of the TCA -treated sample that allows the rebinding of mostly 33 kDa polypeptide restores Y D+, showing that the loss of the EPR signal IIslow| takes place after the removal of 33 kD a polypeptide but not the release of manganese (Mn). Additionally, treatment of several halogenated acetates on Tris-washed PSII particles shows that the degree of their effects on suppressing EPR signal IIslow is correlated with their hydrophobicity. It is postulated that Y D becomes more accessible to some small hydrophobic molecules depending upon their hydrophobicity when 33 kDa poly­peptide is removed.

Fluorescence Decay Kinetics of Wild Type and D2-H117N Mutant Photosystem II Reaction Centers Isolated fromChlamydomonas reinhardtii

2000 ◽  
Vol 104 (19) ◽  
pp. 4777-4781 ◽  
Author(s):  
Heather G. Johnston ◽  
Jun Wang ◽  
Stuart V. Ruffle ◽  
Richard T. Sayre ◽  
Terry L. Gustafson
Keyword(s):  
Photosystem Ii ◽  
Fluorescence Decay ◽  
Reaction Centers ◽  
Wild Type ◽  
Decay Kinetics ◽  
Fluorescence Decay Kinetics ◽  
Kinetics Of
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