Fluorescence decay kinetics of mutants of corn deficient in photosystem I and photosystem II

1984 ◽  
Vol 767 (3) ◽  
pp. 574-581 ◽  
Author(s):  
Beverley R. Green ◽  
Kerry K. Karukstis ◽  
Kenneth Sauer
Keyword(s):  
Photosystem Ii ◽  
Photosystem I ◽  
Fluorescence Decay ◽  
Decay Kinetics ◽  
Fluorescence Decay Kinetics ◽  
Kinetics Of

Fluorescence Decay Kinetics of Wild Type and D2-H117N Mutant Photosystem II Reaction Centers Isolated fromChlamydomonas reinhardtii

2000 ◽  
Vol 104 (19) ◽  
pp. 4777-4781 ◽  
Author(s):  
Heather G. Johnston ◽  
Jun Wang ◽  
Stuart V. Ruffle ◽  
Richard T. Sayre ◽  
Terry L. Gustafson
Keyword(s):  
Photosystem Ii ◽  
Fluorescence Decay ◽  
Reaction Centers ◽  
Wild Type ◽  
Decay Kinetics ◽  
Fluorescence Decay Kinetics ◽  
Kinetics Of

scholarly journals Evidence that D1 Processing Is Required for Manganese Binding and Extrinsic Protein Assembly into Photosystem II

2004 ◽  
Vol 279 (44) ◽  
pp. 45417-45422 ◽  
Author(s):  
Johnna L. Roose ◽  
Himadri B. Pakrasi
Keyword(s):  
Photosystem Ii ◽  
Mutant Cell ◽  
Protein A ◽  
D1 Protein ◽  
Fluorescence Decay ◽  
Normal Function ◽  
Temporal Position ◽  
Decay Kinetics ◽  
Fluorescence Decay Kinetics ◽  
Extrinsic Proteins

Photosystem II (PSII) is a large membrane protein complex that catalyzes oxidation of water to molecular oxygen. During its normal function, PSII is damaged and frequently turned over. The maturation of the D1 protein, a key component in PSII, is a critical step in PSII biogenesis. The precursor form of D1 (pD1) contains a C-terminal extension, which is removed by the protease CtpA to yield PSII complexes with oxygen evolution activity. To determine the temporal position of D1 processing in the PSII assembly pathway, PSII complexes containing only pD1 were isolated from a CtpA-deficient strain of the cyanobacteriumSynechocystis6803. Although membranes from the mutant cell had nearly 50% manganese, no manganese was detected in isolated ΔctpAHT3 PSII, indicating a severely decreased manganese affinity. However, chlorophyll fluorescence decay kinetics after a single saturating flash suggested that the donor YZwas accessible to exogenous Mn2+ions. Furthermore, the extrinsic proteins PsbO, PsbU, and PsbV were not present in PSII isolated from this mutant. However, PsbO and PsbV were present in mutant membranes, but the amount of PsbV protein was consistently less in the mutant membranes compared with the control membranes. We conclude that D1 processing precedes manganese binding and assembly of the extrinsic proteins into PSII. Interestingly, the Psb27 protein was found to be more abundant in ΔctpAHT3 PSII than in HT3 PSII, suggesting a possible role of Psb27 as an assembly factor during PSII biogenesis.

scholarly journals Singlet–triplet annihilation in single LHCII complexes

2015 ◽  
Vol 17 (30) ◽  
pp. 19844-19853 ◽  
Author(s):  
J. Michael Gruber ◽  
Jevgenij Chmeliov ◽  
Tjaart P. J. Krüger ◽  
Leonas Valkunas ◽  
Rienk van Grondelle
Keyword(s):  
Stochastic Model ◽  
Fluorescence Decay ◽  
Decay Kinetics ◽  
Fluorescence Decay Kinetics ◽  
Kinetics Of

The two-exponential fluorescence decay kinetics of single LHCII complexes are quantitatively explained by a stochastic model of singlet–triplet annihilation.

The fluorescence decay kinetics of in vivo chlorophyll measured using low intensity excitation

1979 ◽  
Vol 545 (1) ◽  
pp. 165-174 ◽  
Author(s):  
G.S. Beddard ◽  
G.R. Fleming ◽  
G. Porter ◽  
G.F.W. Searle ◽  
J.A. Synowiec
Keyword(s):  
Fluorescence Decay ◽  
Decay Kinetics ◽  
Low Intensity ◽  
Fluorescence Decay Kinetics ◽  
Kinetics Of
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