Two-dimensional electrophoresis of membrane proteins factors affecting resolution of rat-liver microsomal proteins

1983 ◽  
Vol 734 (2) ◽  
pp. 160-167 ◽  
Author(s):  
E.N.Clare Mills ◽  
Robert B. Freedman
Keyword(s):  
Membrane Proteins ◽  
Rat Liver ◽  
Two Dimensional ◽  
Factors Affecting ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Characterization of microvillar membrane proteins of dog small intestine by two-dimensional electrophoresis

1995 ◽  
Vol 110 (3) ◽  
pp. 483-492 ◽  
Author(s):  
Philip W. Pemberton ◽  
Robert W. Lobley ◽  
Raymond Holmes ◽  
Susanne H. Sørensen ◽  
Kenneth W. Simpson ◽  
...  
Keyword(s):  
Small Intestine ◽  
Membrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of membrane proteins

2007 ◽  
Vol 389 (4) ◽  
pp. 1033-1045 ◽  
Author(s):  
Ralf J. Braun ◽  
Norbert Kinkl ◽  
Monika Beer ◽  
Marius Ueffing
Keyword(s):  
Membrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

scholarly journals Identification of protein disulphide-isomerase as a major acidic polypeptide in rat liver microsomal membranes

1983 ◽  
Vol 213 (1) ◽  
pp. 245-248 ◽  
Author(s):  
E N C Mills ◽  
N Lambert ◽  
R B Freedman
Keyword(s):  
Liver Enzyme ◽  
Rat Liver ◽  
Peptide Mapping ◽  
Bovine Liver ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Protein Disulphide Isomerase ◽  
Two Dimensional Electrophoresis ◽  
Microsomal Membranes ◽  
Dimensional Electrophoresis

Protein disulphide-isomerase was purified to homogeneity from rat liver by a rapid high-yielding procedure. Structural properties of the pure enzyme were very similar to those of the bovine liver enzyme purified by the same method. The purified rat liver enzyme was subjected to two-dimensional gel electrophoresis in the presence and in the absence of microsomal membranes, and shown to co-electrophorese with a major acidic polypeptide clearly identifiable in the two-dimensional electrophoretic profile of microsomal membranes. This identification was confirmed by peptide ‘mapping’ of the pure enzyme and of the defined spot from a two-dimensional electrophoresis gel.

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