Quantitative determination of apolipoprotein A-I in high-density lipoproteins and ‘free’ apolipoprotein A-I by two-dimensional agarose gel lipoprotein-‘rocket’ immunoelectrophoresis of human serum

1986 ◽  
Vol 879 (2) ◽  
pp. 134-139 ◽  
Author(s):  
Wolfgang H. Daerr ◽  
Ute Minzlaff ◽  
Heiner Greten
Keyword(s):  
Quantitative Determination ◽  
Human Serum ◽  
High Density ◽  
High Density Lipoproteins ◽  
Agarose Gel ◽  
Two Dimensional ◽  
Apolipoprotein A

A Mass Spectrometric Determination of the Conformation of Dimeric Apolipoprotein A-I in Discoidal High Density Lipoproteins†

Biochemistry ◽  
2005 ◽  
Vol 44 (24) ◽  
pp. 8600-8607 ◽  
Author(s):  
R. A. Gangani D. Silva ◽  
George M. Hilliard ◽  
Ling Li ◽  
Jere P. Segrest ◽  
W. Sean Davidson
Keyword(s):  
Mass Spectrometric ◽  
High Density ◽  
High Density Lipoproteins ◽  
Mass Spectrometric Determination ◽  
Apolipoprotein A

Immunonephelometric determination of apolipoprotein A-1 in hyperlipoproteinemic serum.

1983 ◽  
Vol 29 (1) ◽  
pp. 120-125 ◽  
Author(s):  
C C Heuck ◽  
I Erbe ◽  
P Flint-Hansen
Keyword(s):  
Scattered Light ◽  
Liquid System ◽  
High Density ◽  
High Density Lipoproteins ◽  
Serum Samples ◽  
Two Phase ◽  
Apolipoprotein A ◽  
One Step ◽  
Phase Liquid

Abstract The intensity of scattered light varies with the size of the scattering particles. Studying the quantitative immunonephelometric determination of apolipoprotein A-1 (apo A-1), we observed that the different particle sizes of lipoproteins must be taken into account in immunonephelometry if apo A-1 is a constituent of the lipoprotein particles. Because interaction between large lipoproteins and immunocomplexes of high-density lipoproteins increases light scattering nonspecifically, false estimations may be obtained in serum with excessive hyperlipoproteinemia. Furthermore, the accessibility of antigenic sites of apo A-1 in intact high-density lipoproteins is limited. Immunonephelometry of apo A-1 in serum necessitates therefore the elimination of various interferents, which we have achieved by a single one-step extraction of lipids in a two-phase liquid system. With n-hexanol/polyfluoro-polychloro-polyethylene, about 90% of the serum lipids are extracted and only apolipoprotein B is precipitated at the interphase. This pretreatment eliminates the interferences caused by excessive hypertriglyceridemia and therefore greatly facilitates the endpoint immunonephelometry of apo A-1 in normal and pathological serum samples.

scholarly journals Importance of Apolipoprotein A-I and A-II Composition in HDL and Its Potential for Studying COVID-19 and SARS-CoV-2

Medicines ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 38
Author(s):  
Kyung-Hyun Cho
Keyword(s):  
Antiviral Activity ◽  
High Density ◽  
High Density Lipoproteins ◽  
Potent Antiviral Activity ◽  
Apolipoprotein A ◽  
Putative Role

The composition and properties of apolipoprotein (apo) A-I and apoA-II in high-density lipoproteins (HDL) might be critical to SARS-CoV-2 infection via SR-BI and antiviral activity against COVID-19. HDL containing native apoA-I showed potent antiviral activity, while HDL containing glycated apoA-I or other apolipoproteins did not. However, there has been no report to elucidate the putative role of apoA-II in the antiviral activity of HDL.

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