A continuous flow method for the study of lipoprotein lipase secretion in adipose cells

1986 ◽  
Vol 875 (2) ◽  
pp. 324-333 ◽  
Author(s):  
Christian Vannier ◽  
Gérard Ailhaud
Keyword(s):  
Lipoprotein Lipase ◽  
Continuous Flow ◽  
Flow Method ◽  
Adipose Cells ◽  
Continuous Flow Method

A stopped-flow/continuous-flow method for kinetic determinations

1995 ◽  
Vol 309 (1-3) ◽  
pp. 277-282 ◽  
Author(s):  
Yun-Sheng Hsieh ◽  
S.R. Crouch
Keyword(s):  
Continuous Flow ◽  
Stopped Flow ◽  
Flow Method ◽  
Continuous Flow Method

ZnO-Based Catalyst for Photodegradation of 2-Chlorophenol in Aqueous Solution Under Simulated Solar Light Using a Continuous Flow Method

JOM ◽  
2020 ◽  
Vol 73 (1) ◽  
pp. 404-410
Author(s):  
Ahed H. Zyoud ◽  
Hala Salah ◽  
Shaher H. Zyoud ◽  
Samer H. Zyoud ◽  
Muath H. Helal ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Continuous Flow ◽  
Solar Light ◽  
Flow Method ◽  
Simulated Solar Light ◽  
Continuous Flow Method

Continuous-flow fluorometry of low galactose concentrations in blood or plasma.

1980 ◽  
Vol 26 (2) ◽  
pp. 282-285 ◽  
Author(s):  
J M Henderson ◽  
F W Fales
Keyword(s):  
Blood Flow ◽  
Hepatic Blood Flow ◽  
Continuous Flow ◽  
Normal Subjects ◽  
Galactose Oxidase ◽  
Fluorogenic Substrate ◽  
Flow Method ◽  
Peroxidase Reaction ◽  
Hydroxyphenylacetic Acid ◽  
Continuous Flow Method

Abstract Clearance of 0-100 mg/L concentrations of galactose from the blood depends on nutrient hepatic blood flow. We can measure such concentrations, which was not previously possible, by a continuous-flow method involving the use of galactose oxidase and peroxidase, the latter being coupled to a fluorogenic substrate, p-hydroxyphenylacetic acid. Interfering substances in the peroxidase reaction are removed by zinc/alkali precipitation. Sensitivity is maximized by using saturating concentrations of the enzymes and substrate. In prepared plasma test samples with galactose concentrations of 10, 40, 70, and 100 mg/L, the within-run CV's ranged from 2.1 to 8.6%, and day-to-day CV's from 2.2 to 17.2%, the largest CV's being for the 10 mg/L concentration. Normal subjects are shown to clear galactose more efficiently than subjects with moderate cirrhosis.

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