High affinity thyroid hormone binding sites on purified rat liver plasma membranes

1977 ◽  
Vol 79 (1) ◽  
pp. 166-172 ◽  
Author(s):  
Nathan B. Pliam ◽  
Ira D. Goldfine
Keyword(s):  
Thyroid Hormone ◽  
Rat Liver ◽  
Binding Sites ◽  
Plasma Membranes ◽  
Hormone Binding ◽  
High Affinity ◽  
Liver Plasma Membranes ◽  
Rat Liver Plasma Membranes

Demonstration of a high affinity Ca2+-ATPase in rat liver plasma membranes

1982 ◽  
Vol 105 (2) ◽  
pp. 488-494 ◽  
Author(s):  
Yasushi Iwasa ◽  
Takafumi Iwasa ◽  
Kenji Higashi ◽  
Kazuo Matsui ◽  
Eishichi Miyamoto
Keyword(s):  
Rat Liver ◽  
Plasma Membranes ◽  
High Affinity ◽  
Liver Plasma Membranes ◽  
Rat Liver Plasma Membranes

Structural characterization of PACAP receptors on rat liver plasma membranes

1993 ◽  
Vol 265 (5) ◽  
pp. G811-G818
Author(s):  
T. D. Nguyen ◽  
G. G. Heintz ◽  
M. S. Wolfe
Keyword(s):  
Rat Liver ◽  
Plasma Membranes ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Membrane Receptors ◽  
Receptor Complexes ◽  
High Affinity ◽  
Liver Plasma Membranes ◽  
Rat Liver Plasma Membranes ◽  
Pacap Receptors

Pituitary adenylate cyclase-activating polypeptide-38 (PACAP-38) and PACAP-27 are recently characterized hypothalamic peptides with marked homology with vasoactive intestinal peptide (VIP), which are concentrated in the innervation of the digestive tract. We now report that, on rat liver plasma membranes, PACAP interacts with at least two types of receptors: receptors demonstrating equally high affinity for PACAP and VIP and receptors with high affinity for PACAP but low affinity for VIP. In contrast, on rat intestinal epithelial cell laterobasal membranes, only receptors with high affinities for PACAP and VIP were observed. After 125I-labeled VIP or 125I-labeled PACAP-27 was cross-linked to the liver plasma membrane receptors with the use of either disuccinimidosuberate or disuccinimido dithiobis(propionate), analysis of the resulting ligand-receptor complexes on sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the structures of the VIP and PACAP receptors were similar: both ligand-receptor complexes displayed two radioactive bands with relative molecular weights of 80,000 and 56,000 under reducing conditions and of 75,000 and 53,000 under nonreducing conditions. These findings suggest that the receptors for the PACAP peptides and VIP are closely related, reflecting the marked homology between these peptides. The presence of receptors specific for PACAP on rat liver plasma membranes should stimulate further studies of the interaction between PACAP and the liver.

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