Partial purification and characterization of two enzymes from guinea-pig liver microsomes that hydrolyze carcinogenic amides 2-acetylaminofluorene and N-hydroxy-2-acetylaminofluorene

1971 ◽  
Vol 20 (11) ◽  
pp. 2971-2982 ◽  
Author(s):  
M. Järvinen ◽  
R.S.S. Santti ◽  
V.K. Hopsu-Havu
Keyword(s):  
Guinea Pig ◽  
Liver Microsomes ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Pig Liver ◽  
Guinea Pig Liver Microsomes ◽  
Guinea Pig Liver

Convenient biosynthetic preparation of isomeric spin-labelled radioactive phosphatidic acids

1982 ◽  
Vol 60 (11) ◽  
pp. 1014-1017 ◽  
Author(s):  
L. Stuhne-Sekalec ◽  
N. Z. Stanacev
Keyword(s):  
Fatty Acids ◽  
Guinea Pig ◽  
Stearic Acid ◽  
Liver Microsomes ◽  
Enzymatic Preparation ◽  
Pig Liver ◽  
Secondary Hydroxyl ◽  
Phosphatidic Acids ◽  
Guinea Pig Liver Microsomes ◽  
Guinea Pig Liver

A convenient method for the enzymatic preparation of sn-3-[2-3H]phosphatidic acids carrying also 5-, 12-, or 16-nitroxide stearic acids, from sn-3-[2-3H]glycerophosphate and isolated guinea pig liver microsomes, is described in detail. The procedure allows a simultaneous preparation of three spin-labelled sn-3-[2-3H] phosphatidic acids of yields 3–3.5 μmol of each compound which is > 99% pure in respect to the radioactivity and which contains 25 mol% of spin-labelled fatty acids. These phosphatidic acids were approximately equally distributed between the primary and the secondary hydroxyl when 12- or 16-nitroxide stearic acids were used or predominantly (75%) associated with the secondary hydroxyl of sn-3-[2-3H]phosphatidic acid when 5-nitroxide stearic acid was present in the incubation mixture.

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