Synaptic transmission between rat spinal cord explants and dissociated superior cervical ganglion neurons in tissue culture

1976 ◽  
Vol 117 (3) ◽  
pp. 437-460 ◽  
Author(s):  
Chien-Ping Ko ◽  
Harold Burton ◽  
Richard P. Bunge
1976 ◽  
Vol 117 (3) ◽  
pp. 461-485 ◽  
Author(s):  
Chien-Ping Ko ◽  
Harold Burton ◽  
Mary I. Johnson ◽  
Richard P. Bunge

1998 ◽  
Vol 274 (3) ◽  
pp. R873-R878 ◽  
Author(s):  
Deborah H. Damon

Endothelin (ET) is a peptide originally identified as an endothelial-derived vasoconstrictor. It is now recognized that ET is produced by and acts on many other tissues including the brain and spinal cord, where it is believed to modulate neurotransmission. The present studies demonstrate that ET is synthesized by and secreted from postganglionic sympathetic neurons. With the use of Northern analysis, ET-1 mRNA was detected in cultures of sympathetic superior cervical ganglion (SCG) neurons isolated from 3- to 5-day old rat pups. ET-1 and ET-3 peptides were also detected in cultured SCG neurons using immunohistochemistry. ET-1 (50 pg/106 cells) and ET-3 (173 pg/106 cells) were detected by radioimmunoassay of media conditioned by cultured SCG. ET-1 (77 pg/mg protein) and ET-3 (30 pg/mg protein) were also detected by radioimmunoassay of extracts of adult SCG.


2011 ◽  
Vol 500 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Joanna Wojtkiewicz ◽  
Judyta K. Juranek ◽  
Ireneusz Kowalski ◽  
Marek Bladowski ◽  
Jarosław Całka ◽  
...  

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