Antigen-specific augmentation of murine immediate hypersensitivity-like footpad reaction by a T-cell factor in the culture supernatant of immune spleen cells

1985 ◽  
Vol 92 (2) ◽  
pp. 350-358 ◽  
Author(s):  
Akira Yamada ◽  
Kunisuke Himeno ◽  
Yoshio Kumazawa ◽  
Kikuo Nomoto
Keyword(s):  
T Cell ◽  
Culture Supernatant ◽  
Immediate Hypersensitivity ◽  
Spleen Cells ◽  
T Cell Factor ◽  
Immune Spleen Cells

scholarly journals A reexamination of the role of LYT-2-positive T cells in murine skin graft rejection.

1984 ◽  
Vol 159 (1) ◽  
pp. 57-67 ◽  
Author(s):  
L LeFrancois ◽  
M J Bevan
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Immune Cells ◽  
Graft Rejection ◽  
Skin Grafts ◽  
Complete Elimination ◽  
Spleen Cells ◽  
Relative Contribution ◽  
Immune Spleen Cells

We have investigated which T cell subclass defined by cytolysis with monoclonal anti-Lyt-1.2 and anti-Lyt-2.2 antibodies is required to adoptively transfer the ability to reject skin grafts. B6.Thy-1.1 spleen cells immune to graft antigens were fractionated with antibody plus C' and transferred to adult thymectomized, irradiated, bone marrow-reconstituted (ATXBM) B6.Thy-1.2 hosts that were simultaneously grafted with BALB.B skin. We found that when the ATXBM hosts were used 6 wk after irradiation and marrow reconstitution, both Lyt-1-depleted and Lyt-2-depleted immune spleen cells could transfer the ability to promptly reject skin grafts. However, such ATXBM recipients of Lyt-2-depleted cells that had rejected skin grafts were found to contain graft-specific CTL that were largely of host (B6.Thy-1.2) origin. When ATXBM hosts were used for the experiment 1 wk after irradiation and marrow reconstitution, no host-derived graft-specific CTL could be detected. However, graft rejection occurred in recipients of anti-Lyt-1- or anti-Lyt-2 plus C'-treated immune cells and specific CTL were generated from spleen cells of both groups. Thus, in the absence of a host-derived response, adoptively transferred immune Lyt-2+ cells, either resistant to, or that escaped from, antibody plus C' treatment, are able to expand in response to the antigenic stimulus provided by the graft. A more complete elimination of specific T cell subclasses is therefore needed to assess the relative contribution of a particular subset to the graft rejection process.

Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations

1991 ◽  
Vol 34 (2) ◽  
pp. 103-110 ◽  
Author(s):  
P. A. Steerenberg ◽  
E. Geerse ◽  
W. H. De Jong ◽  
R. Burger ◽  
R. J. Scheper ◽  
...  
Keyword(s):  
T Cell ◽  
Adoptive Transfer ◽  
Spleen Cells ◽  
Tumour Rejection ◽  
Cell Subpopulations ◽  
T Cell Subpopulations ◽  
Immune Spleen Cells

scholarly journals Antigen-specific suppressor T-cell activity in genetically restricted immune spleen cells.

1978 ◽  
Vol 148 (5) ◽  
pp. 1271-1281 ◽  
Author(s):  
C W Pierce ◽  
J A Kapp
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Activity ◽  
Antibody Responses ◽  
Spleen Cells ◽  
Suppressor T Cells ◽  
Specific Suppressor T Cells ◽  
Immune Spleen Cells

Virgin spleen cells develop comparable primary antibody responses in vitro to syngeneic or allogeneic macrophages (Mphi) bearing the terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT), whereas immune spleen cells primed with syngeneic or allogeneic GAT-Mphi develop secondary responses preferentially when stimulated with GAT-Mphi syngeneic to the GAT-Mphi used for priming in vivo. These restrictions are mediated by products of the I-A subregion of the H-2 complex and are operative at the level of the GAT-Mphi-immune helper T-cell interactions. To investigate why these immune spleen cells fail to develop a significant antibody response to GAT-Mphi other than those used for in vivo immunization and determine the mechanism by which the restriction is maintained, spleen cells from virgin and syngeneic or allogeneic GAT-Mphi-primed mice were co-cultured in the presence of GAT-Mphi of various haplotypes. Antibody responses to GAT developed only in the presence of GAT-Mphi syngeneic to the Mphi used for in vivo priming; responses in cultures with GAT-Mphi allogeneic to the priming Mphi, whether these Mphi were syngeneic or allogeneic with respect to the responding spleen cells, were suppressed. The suppression was mediated by GAT-specific radiosensitive T cells. Thus, development of GAT-specific suppressor T cells appears to be a natural consequence of the immune response to GAT in responder as well as nonresponder mice. The implications of stimulation of genetically restricted immune helper T cells, and antigen-specific, but unrestricted, suppressor T cells after immunization with GAT-Mphi in vivo are discussed in the context of regulatory mechanisms in antibody responses.

scholarly journals Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

1975 ◽  
Vol 142 (5) ◽  
pp. 1241-1253 ◽  
Author(s):  
T Takemori ◽  
T Tada
Keyword(s):  
T Cell ◽  
Antibody Response ◽  
Gel Filtration ◽  
Donor Strain ◽  
Spleen Cells ◽  
Protein Antigens ◽  
Igg Antibody ◽  
Suppressor Factor ◽  
T Cell Factor

An antigen-specific suppressive T-cell factor was extracted from physically disrupted thymocytes and spleen cells of mice that had been immunized with soluble protein antigens. The factor, when inoculated into syngeneic normal mice, could induce a significant suppression of IgG antibody response against a hapten coupled to the carrier protein by which the donor of the suppressor factor was immunized. The suppressor factor was found only effective in suppressing the antibody response of syngeneic or H-2 histocompatible recipients. The suppressive T-cell factor was removed by absorption with immunoadsorbent composed of the relevant antigen, but not with any of those of anti-immunoglobulin antibodies. The factor was successfully removed by alloantibodies with specificity for the K end (H-2K, I-A and I-B) of the H-2 complex of the donor strain, but not by those for the D end (I-C, SsSlp, and H-2D). The activity was removed by absorption with a heterologous antithymocyte serum. The mol wt of the suppression T-cell factor was between 35,000 and 60,000 as determined by Sephadex G-200 gel filtration. The suppressive T-cell factor was found to be a heat-liable protein.

scholarly journals Suppression of antibody and T cell proliferative responses to L-glutamic acid60-L-alanine30-L-tyrosine10 by a specific monoclonal T cell factor.

1980 ◽  
Vol 152 (1) ◽  
pp. 235-240 ◽  
Author(s):  
J A Kapp ◽  
B A Araneo ◽  
B L Clevinger
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
T Cell ◽  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Spleen Cells ◽  
Suppressor T Cells ◽  
Suppressor Factor ◽  
T Cell Factor ◽  
Specific Suppressor T Cells

The synthetic terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) stimulates GAT-specific suppressor T cells in nonresponder mice. Extracts from these T cells contain a GAT-specific soluble T cell suppressor factor (GAT-TsF) that inhibits development of GAT-specific plaque-forming cell (PFC) responses by spleen cells from nonresponder mice stimulated with GAT complexed to methylated bovine serum albumin (GAT-MBSA). These extracts also contain a factor that inhibits development of GAT-specific proliferative responses by GAT-MBSA-primed, nonresponder lymph node T cells. Experiments reported in this manuscript show that a hybrid T cell line, produced by fusion of the AKR thymoma, BW5147, with spleen cells that contain GAT-specific suppressor T cells, produces a constitutive GAT-specific suppresor factor that functionally and serologically resembles GAT-TsF extracted from T cells. More importantly, both GAT-specific PFC and T cell proliferative responses are inhibited by this factor.

scholarly journals THYMUS-DERIVED LYMPHOCYTES PRODUCE AN IMMUNOLOGICALLY SPECIFIC MACROPHAGEARMING FACTOR

1972 ◽  
Vol 136 (5) ◽  
pp. 1318-1322 ◽  
Author(s):  
R. Evans ◽  
C. K. Grant ◽  
Helen Cox ◽  
Kathleen Steele ◽  
P. Alexander
Keyword(s):  
Molecular Weight ◽  
Bone Marrow ◽  
T Cell ◽  
Tumor Cells ◽  
Whole Body ◽  
Target Cells ◽  
Spleen Cells ◽  
Specific Tumor ◽  
Immune Spleen Cells ◽  
Cytophilic Antibody

Spleen cells from mice immunized with an allogeneic tumor when cultured with the specific tumor cells release into the supernatant a specific macrophage-arming factor(s) (SMAF) which binds nonspecifically to macrophages from both mice and rats and renders these cytotoxic to the specific tumor cells. SMAF also binds in an immunologically specific way to the target cells. SMAF-treated target cells grow normally in the absence of macrophages but are killed in the presence of normal macrophages. Thymus-derived cells are necessary for the production of SMAF since (a) after treatment with anti-θ serum immune spleen cells fail to release SMAF; (b) spleen cells from immunized T cell-deprived mice (thymectomized as adults followed by whole body irradiation and restored with bone marrow) fail to produce SMAF on stimulation with the specific target cells. While SMAF has the properties of a cytophilic antibody, it does not belong to one of the established classes of immunoglobulin since high activity is found after column separation in a fraction having a molecular weight between 50,000–60,000 daltons.

scholarly journals Monoclonal alloantibodies specific for the constant region of T cell antigen receptors.

1982 ◽  
Vol 156 (3) ◽  
pp. 888-897 ◽  
Author(s):  
T Tokuhisa ◽  
Y Komatsu ◽  
Y Uchida ◽  
M Taniguchi
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
Antigen Binding ◽  
Constant Region ◽  
Antigen Receptors ◽  
Spleen Cells ◽  
Genetic Studies ◽  
T Cell Factor ◽  
The Right ◽  
Variable Genes

We established three distinct monoclonal antibodies (7C5, 7D1; IgM and 6A4; IgG1) by the fusion of P3U1 and BALB/c (Igh-1a) spleen cells hyperimmunized with T cell blasts from the immunoglobulin heavy chain (Igh) allotype congenic CB-20 (Igh-1b) mice. The 7C5 or 6A4 antibody reacts with the constant region determinants on the antigen-binding molecule (Ct) of the antigen-specific suppressor T cell factor (TsF) or augmenting T cell factor (TaF), respectively. The 7D1 antibody, however, recognizes the shared determinants on the Ct molecules of TsF and TaF. Genetic studies of determinants recognized by these monoclonal antibodies have also suggested that the distinct Ct molecules of TsF and TaF are encoded by two discrete genes linked to the Igh-1b genes, which are located on the right side of the variable genes of Igh on the 12th chromosome. By using the immunoadsorbent columns of 6A4 antibody and anti-I-Ab, TaF, in a manner similar to TsF, was demonstrated to be composed of two chains, i.e., the Ct molecules and the I-A-encoded products. Furthermore, the Ct-bearing molecules were shown to possess the antigen-binding moiety.

scholarly journals Virus and trinitrophenol hapten-specific T-cell-mediated cytotoxicity against H-2 incompatible target cells.

1976 ◽  
Vol 143 (4) ◽  
pp. 999-1004 ◽  
Author(s):  
K Pfizenmaier ◽  
A Strazinski-Powitz ◽  
H Rodt ◽  
M Röllinghoff ◽  
H Wagner
Keyword(s):  
T Cell ◽  
Self Concept ◽  
Target Cells ◽  
Spleen Cells ◽  
Radiation Chimeras ◽  
Cytotoxic Responses ◽  
Immune Spleen Cells

Immune spleen cells from LCM virus-infected (CBA X C57BL/6)F1 radiation chimeras entirely repopulated with CBA-T6 lymphocytes were cytotoxic for allogeneic, LCM virus infected C57BL/6 mouse-derived target cells. Normal C57BL/6 targets were not lysed. CBA-T6 lymphocytes derived from (CBA X C57BL/6) radiation chimeras sensitized in vitro against TNP-conjugated C57BL/6 spleen cells lysed TNP-conjugated C57BL/6 targets. However normal C57BL/6 mouse-derived targets were not destroyed. The magnitude of virus-specific (or TNP-specific) cytotoxic responses against H-2 incompatible targets was lower compared to that against H-2 compatible targets. These data are considered to support and to extend the altered self concept, but are not consistent with the dual recognition concept.

Cytokines produced by T cell subset-depleted naive and listeria-immune spleen cells

1992 ◽  
pp. 585-592
Author(s):  
M. E. A. Mielke ◽  
C. Peters ◽  
S. Brocke ◽  
H. Hahn
Keyword(s):  
T Cell ◽  
Cell Subset ◽  
Spleen Cells ◽  
T Cell Subset ◽  
Immune Spleen Cells
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