A fluorometric method combined with thin layer chromatography for the determination of norepinephrine, epinephrine and dopamine in human urine

1972 ◽  
Vol 36 (2) ◽  
pp. 369-378 ◽  
Author(s):  
Saburo Takahashi ◽  
Leiv R. Gjessing
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Urine ◽  
Fluorometric Method ◽  
Layer Chromatography

Fluorometric Determination of Plasma 11-Hydroxycorticosteroids. II. Studies on the Specificity of the Method

1973 ◽  
Vol 19 (7) ◽  
pp. 718-724 ◽  
Author(s):  
Luis E Mejer ◽  
Roberta C Blanchard
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Total Cholesterol ◽  
Thin Layer Chromatography ◽  
Plasma Cortisol ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Main Component ◽  
Layer Chromatography

Abstract We have investigated the specificity of the fluorometric method we proposed [Clin. Chem. 19, 710 (1973)] for plasma or serum 11-hydroxycorticosteroid determinations. The principal specific fluorogenic contaminants in the cortisol-containing extract of plasma, as detected by thin-layer chromatography and chemically, were triglycerides and total cholesterol. These contaminants contributed an average of 1.6 µg/dl to the total normal cortisol value. Fatty acids were also found, but did not fluoresce. Nonspecific serum fluorogens were quantitated at 1.3 ± 1.2 µg/dl. Cortisol, corticosterone, and fluorogenic contaminants represented an average of 62.0, 29.1, and 9.9%, respectively, of the total fluorometric plasma cortisol value obtained (expressed in terms of cortisol standard). A ratio of 8.3 ± 2.4 was found for cortisol/corticosterone when each component was determined in terms of its respective standard. Fluorescence scans of the plasma cortisol extract indicated cortisol to be the main component present, accompanied by minor fluorescent contaminants.

Fluorometric and Thin Layer Chromatographic Determination of Aminacrine and Its Salts in Drug Preparations

1966 ◽  
Vol 49 (4) ◽  
pp. 837-840
Author(s):  
Laura A Roberts
Keyword(s):  
Carbon Tetrachloride ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Thin Layer Chromatography ◽  
Chromatographic Determination ◽  
Basic Solution ◽  
Antimony Pentachloride ◽  
Fluorometric Method ◽  
Layer Chromatography

Abstract A fluorometric method has been developed for the quantitative determination of aminacrine (USAN name for 9-aminoacridine) and its salts in a wide variety of drug preparations. Aminacrine is extracted with chloroform from basic solution, the chloroform is evaporated, and the residue is dissolved in acidic ethanol. Aminacrine is identified by thin layer chromatography; travel of sample and standard spots is compared under ultraviolet light. Aminacrine is confirmed by spraying the plate with a solution of antimony pentachloride in carbon tetrachloride to produce a yellow spot.

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