A simple micromethod for the direct determination of the total carbon dioxide content in biological fluids

1977 ◽  
Vol 76 (2) ◽  
pp. 161-169 ◽  
Author(s):  
G.J. Van Stekelenburg ◽  
M.H. De Keyzer ◽  
R.A.M.C. Donckerwolcke ◽  
A.H.J. Maas ◽  
R.A.M. van de Camp
Author(s):  
G J Van Stekelenburg ◽  
C Valk ◽  
M J G Van Wijngaarden-Penterman

For those clinical laboratories equipped with a microprocessor-controlled gas analyser, an extremely simple method is described for the determination of the total carbon dioxide content in various biological fluids. Since this method needs only 20 μL of blood plasma or is less dependent on the original total carbon dioxide content, it is especially suited for paediatric purposes. With our procedure the time necessary for one determination equals the time for one capillary blood gas analysis.


1985 ◽  
Vol 9 (4) ◽  
pp. 420-421
Author(s):  
B. Canaud ◽  
A. Araujo ◽  
Q. V. Nguyen ◽  
J. L. Flavier ◽  
C. Mion

1959 ◽  
Vol 14 (3) ◽  
pp. 353-356 ◽  
Author(s):  
Robert C. Stroud

Direct measurements of the ventilatory response to variations in CO2 tension independent of changes in O2 tension are easily obtained by breathing mixtures of carbon dioxide in 100% oxygen. Direct determination of the respiratory response to O2, however, is not possible due to alternations in CO2 tension resulting from changes in ventilation. Therefore, an attempt has been made to determine this response empirically by a combined analysis of breath-holding and ventilatory data. Comparison of experimentally determined responses to various combinations of O2 and CO2 tensions agree quite well with those predicted by this approach and indicate that oxygen plays a small but definite role in the regulation of eupnic breathing at sea level. Submitted on October 22, 1958


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