Sensitive solid phase enzyme immunoassay for human IgA, secretory IgA, and secretory component

1981 ◽  
Vol 116 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Yukio Ishiguro ◽  
Kanefusa Kato ◽  
Takahiro Ito
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Secretory Component ◽  
Secretory Iga

Column enzyme immunoassay for secretory immunoglobulin A in serum.

1983 ◽  
Vol 29 (1) ◽  
pp. 151-153 ◽  
Author(s):  
R Yamamoto ◽  
S Kimura ◽  
S Hattori ◽  
Y Ishiguro ◽  
K Kato
Keyword(s):  
Enzyme Immunoassay ◽  
Sodium Carbonate Solution ◽  
Solid Phase ◽  
Immunoglobulin A ◽  
Enzyme Reaction ◽  
Secretory Component ◽  
Secretory Immunoglobulin A ◽  
Serum Samples ◽  
Sepharose 4B ◽  
Secretory Immunoglobulin

Abstract This enzyme immunoassay for specific measurement of secretory immunoglobulin A concentrations in human serum involves use of a small chromatographic column as a solid-phase. Serum samples are incubated for 2 h with beta-D-galactosidase-labeled antibody to secretory component, then passed through a 0.1-mL Sepharose 4B column containing antibodies to human immunoglobulin A. After the column is washed to remove the unbound label, the buffer in the column is replaced by a solution of o-nitrophenyl-beta-D-galactoside (a beta-D-galactosidase substrate) and incubated at 25 degrees C overnight. The enzyme reaction is stopped by washing the column with sodium carbonate solution, and the absorbance of the eluate is measured at 420 nm. The concentration of secretory immunoglobulin A can be determined with a minimum detectable sensitivity of 3 mg/L, without interference from free immunoglobulin A and secretory component in the same samples.

Separation-free dual solid phase enzyme immunoassay for macromolecules

1988 ◽  
Vol 60 (4) ◽  
pp. 353-356 ◽  
Author(s):  
Keith J. Schray ◽  
Raymond S. Niedbala
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase

Development of a Solid Phase Enzyme Immunoassay for the Detection of Anti-Ro Autoantibodies

1991 ◽  
Vol 20 (4) ◽  
pp. 267-273 ◽  
Author(s):  
T. Dörner ◽  
F. Hiepe ◽  
F. Mielke ◽  
S. T. Kiessig ◽  
A. Lukowsky ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase

scholarly journals A solid-phase enzyme immunoassay using guinea pig C3 to detect anti-Mycoplasma pulmonis antibody in the sera of infected rats.

1988 ◽  
Vol 36 (5) ◽  
pp. 1803-1807
Author(s):  
NORIAKI KOBAYASHI ◽  
EIJI TERADA ◽  
YOSHINORI SAKURAI ◽  
HIROKAZU SUZUKI ◽  
AKIRA TAKAKURA ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Mycoplasma Pulmonis

Solid-phase enzyme immunoassay for determination of antibodies to cytomegalovirus.

1980 ◽  
Vol 12 (1) ◽  
pp. 46-51 ◽  
Author(s):  
R V Krishna ◽  
O H Meurman ◽  
T Ziegler ◽  
U H Krech
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase
Sign in / Sign up

Export Citation Format

Share Document