Pattern of binding of tritiated actinomycin D to Phaseolus coccineus polytene chromosomes

1972 ◽  
Vol 75 (1) ◽  
pp. 154-158 ◽  
Author(s):  
P.G. Cionini ◽  
S. Avanzi
Keyword(s):  
Actinomycin D ◽  
Polytene Chromosomes ◽  
Phaseolus Coccineus

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

Cytological localization of ribosomal cistrons in polytene chromosomes of Phaseolus coccineus

Chromosoma ◽  
1972 ◽  
Vol 39 (2) ◽  
pp. 191-203 ◽  
Author(s):  
S. Avanzi ◽  
M. Durante ◽  
P. G. Cionini ◽  
F. D'Amato
Keyword(s):  
Polytene Chromosomes ◽  
Phaseolus Coccineus ◽  
Ribosomal Cistrons ◽  
Cytological Localization

Application of 7-amino-actinomycin D for the fluorescence microscopical analysis of DNA in cells and polytene chromosomes

1985 ◽  
Vol 17 (2) ◽  
pp. 131-142 ◽  
Author(s):  
Natalia G. Stepanova ◽  
Sergei M. Nikitin ◽  
Flora S. Valeeva ◽  
Olga N. Kartasheva ◽  
Alexey L. Zhuze ◽  
...  
Keyword(s):  
Actinomycin D ◽  
Polytene Chromosomes ◽  
Microscopical Analysis ◽  
In Cells

scholarly journals RNA SYNTHESIS BY EXOGENOUS RNA POLYMERASE ON CYTOLOGICAL PREPARATIONS OF CHROMOSOMES

1973 ◽  
Vol 57 (2) ◽  
pp. 538-550 ◽  
Author(s):  
R. Sederoff ◽  
R. Clynes ◽  
M. Poncz ◽  
S. Hachtel
Keyword(s):  
Rna Polymerase ◽  
Dna Sequences ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Polytene Chromosomes ◽  
Polymerase Activity ◽  
Exogenous Rna ◽  
Rna Polymerase Activity ◽  
Chromosome Regions

Cytological preparations of Drosophila polytene chromosomes serve as templates for RNA synthesis carried out by exogenous RNA polymerase (Escherichia coli). Incorporation of labeled ribonucleoside triphosphates into RNA may be observed directly by autoradiography. Because of the effects of rifampicin, actinomycin D, ribonuclease, high salt, and the requirement for all four nucleoside triphosphates, we conclude that the labeling observed over chromosomes is due to DNA-dependent RNA polymerase activity. Using this method, one can observe RNA synthesis in vitro on specific chromosome regions due to the activity of exogenous RNA polymerase. We find that much of the RNA synthesis in this system occurs on DNA sequences which appear to be in a nondenatured state.

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