Babesia bovis: Purification and concentration of merozoites and infected bovine erythrocytes

1986 ◽  
Vol 61 (2) ◽  
pp. 236-243 ◽  
Author(s):  
S.D. Rodriguez ◽  
G.M. Buening ◽  
C.A. Vega ◽  
C.A. Carson
Keyword(s):  
Babesia Bovis ◽  
Bovine Erythrocytes

In vitro adherence of bovine erythrocytes infected with Babesia bovis to thrombospondin and laminin

1989 ◽  
Vol 19 (5) ◽  
pp. 567-569 ◽  
Author(s):  
F. Parrodi ◽  
I.G. Wright ◽  
A.S. Bourne ◽  
C. Dobson
Keyword(s):  
Babesia Bovis ◽  
Bovine Erythrocytes

scholarly journals Cellular Localization of Babesia bovis Merozoite Rhoptry-Associated Protein 1 and Its Erythrocyte-Binding Activity

2002 ◽  
Vol 70 (10) ◽  
pp. 5822-5826 ◽  
Author(s):  
Naoaki Yokoyama ◽  
Boonchit Suthisak ◽  
Haruyuki Hirata ◽  
Tomohide Matsuo ◽  
Noboru Inoue ◽  
...  
Keyword(s):  
Host Cell ◽  
Binding Affinity ◽  
Cellular Localization ◽  
Early Stage ◽  
Babesia Bovis ◽  
Host Cells ◽  
Host Cell Membrane ◽  
Erythrocyte Binding ◽  
Bovine Erythrocytes

ABSTRACT The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent antibody test, RAP-1 was detectable in all developmental stages of merozoites and in extracellular merozoites. In the early stage of merozoite development, RAP-1 appears as a dense accumulation, which later thins out and blankets the host cell cytoplasm, but retains a denser mass around newly formed parasite nuclei. The preferential accumulations of RAP-1 on the inner surface of a host cell membrane and bordering the parasite's outer surface were demonstrable by immunoelectron microscopy. An erythrocyte-binding assay with the lysate of merozoites demonstrated RAP-1 binding to both bovine and equine erythrocytes. Anti-RAP-1 monoclonal antibody 1C1 prevented the interaction of RAP-1 with bovine erythrocytes and significantly inhibited parasite proliferation in vitro. With the recombinant RAP-1, the addition of increasing concentrations of Ca2+ accentuated its binding affinity with bovine erythrocytes. The present findings lend support to an earlier proposition of an erythrocytic binding role for RAP-1 expressed in B. bovis merozoites and, possibly, its involvement in the escape of newly formed merozoites from host cells.

Development of a solid-phase radioimmunoassay for antibody to antigens of Babesia bovis infected bovine erythrocytes

1982 ◽  
Vol 12 (2-3) ◽  
pp. 103-109 ◽  
Author(s):  
L.P. Kahl ◽  
R.F. Anders ◽  
L.L. Callow ◽  
B.J. Rodwell ◽  
G.F. Mitchell
Keyword(s):  
Solid Phase ◽  
Babesia Bovis ◽  
Solid Phase Radioimmunoassay ◽  
Bovine Erythrocytes

Babesia bovis: Biosynthesis and localisation of the 12D3 antigen in bovine erythrocytes

1996 ◽  
Vol 26 (11) ◽  
pp. 1255-1262 ◽  
Author(s):  
G.S. Harper ◽  
A.R. Hibbs ◽  
I.J. East ◽  
D.J. Waltisbuhl ◽  
W.K. Jorgensen ◽  
...  
Keyword(s):  
Babesia Bovis ◽  
Bovine Erythrocytes

scholarly journals Calcium ions are involved in egress of Babesia bovis merozoites from bovine erythrocytes

2015 ◽  
Vol 77 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Ehab MOSSAAD ◽  
Masahito ASADA ◽  
Daichi NAKATANI ◽  
Noboru INOUE ◽  
Naoaki YOKOYAMA ◽  
...  
Keyword(s):  
Calcium Ions ◽  
Babesia Bovis ◽  
Bovine Erythrocytes

scholarly journals Babesia bovis Merozoite Surface Antigen 2 Proteins Are Expressed on the Merozoite and Sporozoite Surface, and Specific Antibodies Inhibit Attachment and Invasion of Erythrocytes

2002 ◽  
Vol 70 (11) ◽  
pp. 6448-6455 ◽  
Author(s):  
Juan Mosqueda ◽  
Terry F. McElwain ◽  
Guy H. Palmer
Keyword(s):  
Surface Antigen ◽  
Significant Inhibition ◽  
Babesia Bovis ◽  
Merozoite Invasion ◽  
Specific Antibodies ◽  
Surface Molecules ◽  
Erythrocyte Invasion ◽  
Merozoite Surface Antigen ◽  
Initial Binding ◽  
Bovine Erythrocytes

ABSTRACT The Babesia bovis merozoite surface antigen 2 (MSA-2) locus encodes four proteins, MSA-2a1, -2a2, -2b, and -2c. With the use of specific antibodies, each MSA-2 protein was shown to be expressed on the surface of live extracellular merozoites and coexpression on single merozoites was confirmed. Individual antisera against MSA-2a, MSA-2b, and MSA-2c significantly inhibited merozoite invasion of bovine erythrocytes. As tick-derived sporozoites also directly invade erythrocytes, expression of each MSA-2 protein on the sporozoite surface was examined and verified. Finally, statistically significant inhibition of sporozoite binding to the erythrocytes was demonstrated by using antisera specific for MSA-2a, MSA-2b, and MSA-2c. These results indicate an important role for MSA-2 proteins in the initial binding and invasion of host erythrocytes and support the hypothesis that sporozoites and merozoites use common surface molecules in erythrocyte invasion.

Amount of cholesterol in host membrane affects erythrocyte invasion and replication by Babesia bovis

Parasitology ◽  
2006 ◽  
Vol 134 (5) ◽  
pp. 625-630 ◽  
Author(s):  
K. OKUBO ◽  
N. YOKOYAMA ◽  
N. TAKABATAKE ◽  
M. OKAMURA ◽  
I. IGARASHI
Keyword(s):  
Erythrocyte Membrane ◽  
Babesia Bovis ◽  
Cholesterol Depletion ◽  
Slight Reduction ◽  
Erythrocyte Invasion ◽  
Host Membrane ◽  
Growth Assay ◽  
Pre Treatment ◽  
Bovine Erythrocytes

SUMMARYCholesterol is a major component of the erythrocyte membrane. In the present study, we investigated the effects of cholesterol reduction in host bovine erythrocytes (RBC) on the growth of Babesia bovis, a major bovine haemoprotozoon. An in vitro growth assay with bovine RBC that had been prepared by pre-treatment with a cholesterol depletion agent (methyl-β-cyclodextrin, MCD) showed that the culture with 5 mm MCD-treated RBC inhibited the growth of B. bovis significantly as compared with that with the control RBC. In further experiments, the treatment with 5 mm MCD was proved to suppress both activities of the parasite, erythrocyte invasion and replication within the infected RBC. In contrast, a slight reduction in the membrane cholesterol by 1 mm MCD treatment promoted both their growth and erythrocyte invasion activity. These results indicate that erythrocyte invasion and replication by B. bovis are affected by the amount of cholesterol in the host erythrocyte membrane.

scholarly journals Detection of Anaplasma Marginale DNA in Bovine Erythrocytes by Slot-Blot and in Situ Hybridization with a PCR-Mediated Digoxigenin-Labeled DNA Probe

1995 ◽  
Vol 7 (4) ◽  
pp. 465-472 ◽  
Author(s):  
Nie-Lin Ge ◽  
Katherine M. Kocan ◽  
George L. Murphy ◽  
Edmour F. Blouin
Keyword(s):  
In Situ Hybridization ◽  
Acute Infection ◽  
Anaplasma Marginale ◽  
Babesia Bovis ◽  
Tetrazolium Salt ◽  
Slot Blot ◽  
Infected Blood ◽  
Blood Smears ◽  
Bovine Erythrocytes

A 409-base pair (bp) DNA fragment derived from the msp-1β gene of Anaplasma marginale was amplified and simultaneously labeled with digoxigenin-11-dUTP by a polymerase chain reaction (PCR) assay. The resulting digoxigenin-labeled 409-bp PCR product was used as a probe for slot-blot and in situ hybridization to detect A. marginale DNA from experimentally infected bovine erythrocytes. The hybrid formation was detected with alkaline phosphatase-conjugated anti-digoxigenin antibody and substrates 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium salt. In slot-blot hybridizations, the probe detected A. marginale DNA from approximately 1,000-10,000 infected erythrocytes in 1.25 ml of whole blood, which is equivalent to a parasitemia level of 0.00001%. The probe proved to be A. marginale-specific when tested with 17 species of microorganisms. The applicability of the probe for diagnosis was tested by screening A. marginale infections in 2 experimentally infected splenectomized cattle before microscopically detectable parasitemias and after acute infection. After inoculation of infected blood, A. marginale infections were detected with the probe 14 days prior to detection in stained smears. Microscopically inapparent parasitemias were also detected with the probe for 2 months after acute disease. When the probe was used for in situ hybridization on methanol-fixed blood smears, probe reaction could be visualized with light microscopy on A. marginale inclusions within infected erythrocytes. The probe reaction was not observed on leukocytes and uninfected erythrocytes from infected blood smears, on erythrocytes from uninfected blood samples, or on samples infected with A. ovis, Babesia bovis, or B. bigemina. This PCR-mediated nonradioactive probe appears to be a sensitive diagnostic test for A. marginale.

Complement does not facilitate in vitro invasion of bovine erythrocytes by Babesia bovis

1986 ◽  
Vol 80 (3) ◽  
pp. 377-378 ◽  
Author(s):  
M. G. Levy ◽  
I. Kakoma ◽  
G. C. Clabaugh ◽  
M. Ristic
Keyword(s):  
Babesia Bovis ◽  
In Vitro Invasion ◽  
Bovine Erythrocytes
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