Synthesis and prostaglandin E2-induced secretion of surfactant phospholipid by isolated gastric mucous cells

1991 ◽  
Vol 100 (5) ◽  
pp. 1232-1240 ◽  
Author(s):  
James M. Scheiman ◽  
Eugene R. Kraus ◽  
Leslie A. Bonnville ◽  
Paul A. Weinhold ◽  
C.Richard Boland
Keyword(s):  
Prostaglandin E2 ◽  
Mucous Cells ◽  
Gastric Mucous

Synthesis and Prostaglandin E2–Induced Secretion of Surfactant Phospholipid by Isolated Gastric Mucous Cells

1991 ◽  
Vol 100 (5) ◽  
pp. 1232-1240 ◽  
Author(s):  
James M. Scheiman ◽  
Eugene R. Kraus ◽  
Leslie A. Bonnville ◽  
Paul A. Weinhold ◽  
C. Richard Boland
Keyword(s):  
Prostaglandin E2 ◽  
Mucous Cells ◽  
Gastric Mucous

Induction of cyclooxygenase protein and stimulation of prostaglandin E2 release by epidermal growth factor in cultured guinea pig gastric mucous cells

1995 ◽  
Vol 40 (8) ◽  
pp. 1679-1686 ◽  
Author(s):  
Osamu Nakano ◽  
Choitsu Sakamoto ◽  
Kohei Matsuda ◽  
Yoshitaka Konda ◽  
Takashi Matozaki ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Guinea Pig ◽  
Prostaglandin E2 ◽  
Mucous Cells ◽  
Epidermal Growth ◽  
Gastric Mucous ◽  
Stimulation Of

Helicobacter pylori infection produces expression of secretory component in gastric mucous cells

2001 ◽  
Vol 120 (5) ◽  
pp. A708-A709
Author(s):  
T KANEKO ◽  
H OTA ◽  
M HAYAMA ◽  
K NAKAJIMA ◽  
A YOSHIZAWA ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretory Component ◽  
Helicobacter Pylori Infection ◽  
Mucous Cells ◽  
Gastric Mucous

Effect of 16,16-dimethyl prostaglandin E2 on lipidic organelles of rat gastric surface mucous cells

1993 ◽  
Vol 104 (1) ◽  
pp. 103-113 ◽  
Author(s):  
Ya-Chu J. Kao ◽  
Lenard M. Lichtenberger
Keyword(s):  
Prostaglandin E2 ◽  
Mucous Cells

Characterization of mucous cell synthetic functions in a new primary canine gastric mucous cell culture system

1990 ◽  
Vol 258 (5) ◽  
pp. G774-G787 ◽  
Author(s):  
C. R. Boland ◽  
E. R. Kraus ◽  
J. M. Scheiman ◽  
C. Black ◽  
G. D. Deshmukh ◽  
...  
Keyword(s):  
Culture System ◽  
Mucous Cell ◽  
Gastric Mucin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Metabolic Labeling ◽  
Mucous Cells ◽  
Gastric Mucus ◽  
Adequate Model ◽  
Gastric Mucous Cell ◽  
Gastric Mucous

Mucin is a critical component of the protective layer secreted by gastrointestinal mucous cells. A detailed understanding of the molecular processing of gastric mucin and the physiology of its secretion has been limited by the lack of an adequate model for their study. We have developed a primary culture system of canine gastric mucous cells that has permitted us to study their synthetic and secretory functions. It was found that [3H]glucosamine used for metabolic labeling studies was incorporated into both mucin and lipid components of gastric mucus. To measure mucin with this model, a new immunoassay was developed to quantitate canine gastric mucin. Mucin was purified from the canine stomach, a polyclonal antibody was generated, and an enzyme-linked immunosorbent assay for gastric mucin was established. Mast cells were frequent contaminants of the gastric mucous cell preparation, and two methods were developed to limit their contamination. A new culture system has been developed for the study of gastric mucous cells. These cells synthesize and secrete both mucin and phospholipids. This system will permit us to study the molecular processing of mucin and the physiology of its production and release.

Renewal of mouse gastric mucous cells following fast neutron irradiation

1977 ◽  
Vol 183 (3) ◽  
pp. 303-318 ◽  
Author(s):  
W. Wattel ◽  
J. J. Geuze ◽  
D. G. de Rooij ◽  
J. A. G. Davids
Keyword(s):  
Fast Neutron ◽  
Neutron Irradiation ◽  
Mucous Cells ◽  
Fast Neutron Irradiation ◽  
Gastric Mucous
Sign in / Sign up

Export Citation Format

Share Document