Purification and characterization of a calf thymus protein active on lipid metabolism

1989 ◽  
Vol 21 (9) ◽  
pp. 1009-1014 ◽  
Author(s):  
Paolo Mondola ◽  
Mariarosaria Santillo ◽  
Franco Santangelo ◽  
Carlo Caporale ◽  
Anna Belfiore ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Purification And Characterization ◽  
Calf Thymus

scholarly journals Purification and characterization of a poly(A)-specific exoribonuclease from calf thymus.

1980 ◽  
Vol 255 (10) ◽  
pp. 4535-4538
Author(s):  
H.C. Schröder ◽  
R.K. Zahn ◽  
K. Dose ◽  
W.E. Müller
Keyword(s):  
Purification And Characterization ◽  
Calf Thymus

The purification and characterization of deoxycytidine kinase from calf thymus

2008 ◽  
Vol 25 (3) ◽  
pp. 475-480 ◽  
Author(s):  
Jing Quan ◽  
Yan-Qian Chai ◽  
Christopher J. Branford-White ◽  
Li-Min Zhu
Keyword(s):  
Deoxycytidine Kinase ◽  
Purification And Characterization ◽  
Calf Thymus

scholarly journals Purification and Characterization of Mycobacterial Phospholipase A: an Activity Associated with Mycobacterial Cutinase

2007 ◽  
Vol 189 (11) ◽  
pp. 4153-4160 ◽  
Author(s):  
Sarah K. Parker ◽  
Kathryn M. Curtin ◽  
Michael L. Vasil
Keyword(s):  
Fatty Acids ◽  
Mycobacterium Tuberculosis ◽  
Lipid Metabolism ◽  
Reverse Genetics ◽  
Phospholipase A ◽  
Purification And Characterization ◽  
Disease Pathogenesis ◽  
Serine Esterases ◽  
Primary Substrate

ABSTRACT We describe mycobacterial phospholipase A activity (MPLA) and, using reverse genetics, have associated this activity with putative mycobacterial cutinase. PLAs, which hydrolyze fatty acids on phospholipids, play a significant role in human inflammatory states and disease pathogenesis. In prokaryotes, the recognition of their role in virulence is more recent. Cutinases are serine esterases whose primary substrate is cutin, the waxy exterior layer of plants. Mycobacterium tuberculosis has maintained seven putative cutinases, though it should not encounter cutin; we demonstrate that known cutinases and MPLA cleave phospholipids in a PLA-type manner and also hydrolyze Tween. We analyzed cutinase motifs in mycobacteria and found the motif very prevalent. All mycobacteria tested had MPLA activity. These studies suggest an alternative use for putative cutinases by the M. tuberculosis group that is likely related to MPLA activity and lipid metabolism.

Purification and Characterization of the Ca2+ Plus Mg2+-Dependent Endodeoxyribonuclease from Calf Thymus Chromatin1

1981 ◽  
Vol 89 (1) ◽  
pp. 143-152 ◽  
Author(s):  
Michitoshi NAKAMURA ◽  
Yoshiyuki SAKAKI ◽  
Nobuko WATANABE ◽  
Yasuyuki TAKAGI
Keyword(s):  
Purification And Characterization ◽  
Calf Thymus
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