Long-term in vitro cultivation of Plasmodium berghei

1987 ◽  
Vol 17 (7) ◽  
pp. 1329-1331 ◽  
Author(s):  
Mira L. Ramaiya ◽  
Vijayalaxmi R. Kamath ◽  
D.M. Renapurkar
Keyword(s):  
Plasmodium Berghei ◽  
In Vitro Cultivation

Effects of long-term in vitro cultivation on the virulence of cloned lines of Leishmania major promastigotes

1992 ◽  
Vol 86 (4) ◽  
pp. 347-354 ◽  
Author(s):  
M. Segovia ◽  
J. M. Artero ◽  
E. Mellado ◽  
M. L. Chance
Keyword(s):  
Leishmania Major ◽  
In Vitro Cultivation

scholarly journals MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS

1973 ◽  
Vol 138 (2) ◽  
pp. 364-372 ◽  
Author(s):  
M. Hatanaka ◽  
R. Klein ◽  
C. W. Long ◽  
R. Gilden
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
In Vitro Cultivation ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
A Cell ◽  
Phenotypic Reversion

Tumorigenic and nontumorigenic mutants induced by a single 5'-bromodeoxyuridine (BrdU) treatment of a nonproducer (NP) tumorigenic cell line were isolated and characterized. Among the cloned derivatives were examples of virus-free and sarcoma virus-producing cell lines. Oncogenicity did not correlate with production of virus or ease of rescue of the sarcoma genome. All lines, including nononcogenic derivatives, retained the sarcoma genome. Phenotypic reversion of some cell mutants was observed after in vivo inoculation or long term in vitro cultivation. The M-50T cell line, obtained from a tumor induced by M-50 cells, had a sarcoma genome rescuable by direct superinfection; this was only achieved with parental M-50 cells by a cell fusion rescue technique. The M-43-2T cell, obtained from a single small static tumor induced by otherwise nononcogenic M-43-2 cells, shed sarcoma virus and became tumorigenic. M-58-4-48 became tumorigenic after passage 48 of the M-58-4 line, which was originally nontumorigenic. These observations of phenotypic reversion demonstrate that the presence of the sarcoma gene in cells is an essential but not sufficient condition of tumorigenesis.

Poly(dimethylsiloxane)-based microdevices for studying plant reproduction

2014 ◽  
Vol 42 (2) ◽  
pp. 320-324 ◽  
Author(s):  
Hideyuki Arata ◽  
Tetsuya Higashiyama
Keyword(s):  
Pollen Tube ◽  
Experimental Studies ◽  
Plant Reproduction ◽  
Plant Tissues ◽  
In Vitro Cultivation ◽  
Future Prospects ◽  
Experimental Approaches ◽  
Microchannel Device

Long-term holding and precise handling of growing plant tissues during in vitro cultivation has been a major hurdle for experimental studies related to plant development and reproduction. In the present review, we introduce two of our newly developed poly(dimethylsiloxane)-based microdevices: a T-shaped microchannel device for pollen tube chemoattraction and a microcage array for long-term live imaging of ovules. Their design, usage and advantages are described, and future prospects of experimental approaches to plant reproduction using such microdevices are discussed.

Long-term in vitro cultivation of Borrelia burgdorferi sensu lato strains: influence on plasmid patterns, genome stability and expression of proteins

1997 ◽  
Vol 148 (2) ◽  
pp. 109-118 ◽  
Author(s):  
U. Busch ◽  
G. Will ◽  
C. Hizo-Teufel ◽  
B. Wilske ◽  
V. Preac-Mursic
Keyword(s):  
Borrelia Burgdorferi ◽  
Genome Stability ◽  
Borrelia Burgdorferi Sensu Lato ◽  
In Vitro Cultivation

scholarly journals Utilising FGF2, IGF2 and FSH in serum-free protocol for long-term in vitro cultivation of primary human granulosa cells

2020 ◽  
Vol 510 ◽  
pp. 110816
Author(s):  
Kati Hensen ◽  
Martin Pook ◽  
Anu Sikut ◽  
Tõnis Org ◽  
Toivo Maimets ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Cultivation ◽  
Serum Free ◽  
Human Granulosa Cells

In Vitro Cultivation of the Exoerythrocytic Stage of Plasmodium berghei in Irradiated Hepatoma Cells

1985 ◽  
Vol 34 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Michael R. Hollingdale ◽  
Pamela Leland ◽  
Cynthia I. Sigler
Keyword(s):  
Plasmodium Berghei ◽  
Hepatoma Cells ◽  
In Vitro Cultivation

SURVIVAL AND PRODUCTIVITY OF POTATO PLANTS OF LONG-TERM IN VITRO CULTIVATION IN THE CONDITIONS OF SOIL SUBSTRATE

2019 ◽  
Vol 2 (61) ◽  
pp. 28-33
Author(s):  
Saidvali F. Saydalizoda ◽  
◽  
Zarafo S. Kiyomova ◽  
Nigora N. Nazarova ◽  
Kurbon Aliev ◽  
...  
Keyword(s):  
In Vitro Cultivation ◽  
Soil Substrate ◽  
Potato Plants

In Vitro Cultivation of Erythrocytic Forms of Plasmodium knowlesi and Plasmodium berghei

1966 ◽  
Vol 131 (suppl_9) ◽  
pp. 1026-1031 ◽  
Author(s):  
H. Polet
Keyword(s):  
Plasmodium Berghei ◽  
Plasmodium Knowlesi ◽  
In Vitro Cultivation

Use of various animal erythrocytes for in vitro cultivation of Plasmodium berghei

1988 ◽  
Vol 18 (5) ◽  
pp. 639-641 ◽  
Author(s):  
Mira L. Ramaiya ◽  
D.M. Renapurkar
Keyword(s):  
Plasmodium Berghei ◽  
In Vitro Cultivation
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