scholarly journals In Vitro Cultivation of Plasmodium berghei: Application of Drug Resistance Tests with Chloroquine and Artesunate

2013 ◽  
Author(s):  
İpek ÖSTAN ◽  
Özgür KURT ◽  
Ahmet ÖZBİLGİN
Keyword(s):  
Drug Resistance ◽  
Plasmodium Berghei ◽  
In Vitro Cultivation ◽  
Resistance Tests

In Vitro Effect of Chlorquine and Picroliv on Plasmodium Berghei Induced Alterations in the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and Malondialdehyde in spleen Explant Culture

2020 ◽  
Vol 20 ◽  
Author(s):  
Anchal Trivedi ◽  
Aparna Misra ◽  
Esha Sarkar ◽  
Anil K. Balapure
Keyword(s):  
Drug Resistance ◽  
Plasmodium Berghei ◽  
Adenosine Triphosphatase ◽  
Explant Culture ◽  
Need To Evaluate ◽  
Mda Content ◽  
High Level ◽  
Vitro Effect ◽  
Positive Effect

Background: In recent years, great progress has been made in reducing the high level of malaria suffering worldwide. There is a great need to evaluate drug resistance reversers and consider new medicines against malaria. There are many approaches to the development of antimalarial drugs. Specific concerns must be taken in to account in these approaches, in particular there requirement for very in expensive and simple use of new therapies and the need to limit drug discovery expenses. Important ongoing efforts are the optimisation of treatment with available medications, including the use of combination therapy. The production of analogs of known agents and the identification of natural products, the use of compounds originally developed against other diseases, the assessment of overcoming drug resistance and the consideration of new therapeutic targets. Liver and spleen are the important organs which are directly associated with malarial complications. Aim: An analysis the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and Malondialdehyde in spleen Explant Culture. Objective: To determine in-Vitro Effect of Chlorquine and Picroliv on Plasmodium Berghei Induced Alterations in the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and Malondialdehyde in spleen Explant Culture. Material and method: 1-Histological preparation of spleen explants for paraplast embedding 2-Biochemicalstudies (Enzymes (Atpase, ALP&GST) and the level of protein, Malondialdehyde (MDA). Result: Splenomegalyis one of the three main diagnostic parameters of malaria infection besides fever and anaemia. Many enzymes present in the liver and spleen may also be altered or liberated under different pathological conditions. Enzymes (ATPase, ALP&GST) and the level of protein, Malondialdehyde (MDA) content was found to increase in the liver and spleen explants during malarial infection. In the liver and spleen derived from parasitized CQ treated animals, the activity of all the above enzymes (ATPase, ALP&GST) and the level of protein & MDA of liver/spleen reversed towards the normal for all the 4or3 days of incubations. Picroliv efficacy decreased with the increment of parasitaemia and at 60%parasitaemia. Conclusion: Alkalinephosphatase (ALP) was found to increase with increasing parasitaemia. After the addition of Picroliv to the medium, a decrement in the activity was observed up to day 4 of culture.A similar positive effect of Picroliv was observed on the ATPase and ALP activity of spleen explants.DNA and protein contents also increased in the parasitized liver cultured in the presence of picroliv.On the contrary, in the spleen explants DNA, protein and MDA content were found to decrease after Picroliv supplementation to the culture medium.

scholarly journals Plasmodium vivax and Drug Resistance

2021 ◽  
Author(s):  
Puji Budi Setia Asih ◽  
Din Syafruddin
Keyword(s):  
Drug Resistance ◽  
Molecular Basis ◽  
Indirect Evidence ◽  
Antimalarial Drugs ◽  
In Vivo Studies ◽  
In Vitro Cultivation ◽  
Radical Cure ◽  
The Status

Resistance to antimalarial drugs is a threat to global efforts to eliminate malaria by 2030. Currently, treatment for vivax malaria uses chloroquine or ACT for uncomplicated P. vivax whereas primaquine is given to eliminate latent liver stage infections (a method known as radical cure). Studies on P. vivax resistance to antimalarials and the molecular basis of resistance lags far behind the P. falciparum as in vitro cultivation of the P. vivax has not yet been established. Therefore, data on the P. vivax resistance to any antimalarial drugs are generated through in vivo studies or through monitoring of antimalarial treatments in mixed species infection. Indirect evidence through drug selective pressure on the parasites genome, as evidenced by the presence of the molecular marker(s) for drug resistance in areas where P. falciparum and P. vivax are distributed in sympatry may reflect, although require validation, the status of P. vivax resistance. This review focuses on the currently available data that may represent the state-of-the art of the P. vivax resistance status to antimalarial to anticipate the challenge for malaria elimination by 2030.

In Vitro Cultivation of the Exoerythrocytic Stage of Plasmodium berghei in Irradiated Hepatoma Cells

1985 ◽  
Vol 34 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Michael R. Hollingdale ◽  
Pamela Leland ◽  
Cynthia I. Sigler
Keyword(s):  
Plasmodium Berghei ◽  
Hepatoma Cells ◽  
In Vitro Cultivation

Long-term in vitro cultivation of Plasmodium berghei

1987 ◽  
Vol 17 (7) ◽  
pp. 1329-1331 ◽  
Author(s):  
Mira L. Ramaiya ◽  
Vijayalaxmi R. Kamath ◽  
D.M. Renapurkar
Keyword(s):  
Plasmodium Berghei ◽  
In Vitro Cultivation

In Vitro Cultivation of Erythrocytic Forms of Plasmodium knowlesi and Plasmodium berghei

1966 ◽  
Vol 131 (suppl_9) ◽  
pp. 1026-1031 ◽  
Author(s):  
H. Polet
Keyword(s):  
Plasmodium Berghei ◽  
Plasmodium Knowlesi ◽  
In Vitro Cultivation

Use of various animal erythrocytes for in vitro cultivation of Plasmodium berghei

1988 ◽  
Vol 18 (5) ◽  
pp. 639-641 ◽  
Author(s):  
Mira L. Ramaiya ◽  
D.M. Renapurkar
Keyword(s):  
Plasmodium Berghei ◽  
In Vitro Cultivation

In Vitro Cultivation of the Exoerythrocytic Stage of Plasmodium Berghei in a Hepatoma Cell Line

1983 ◽  
Vol 32 (4) ◽  
pp. 682-684 ◽  
Author(s):  
Michael R. Hollingdale ◽  
Pamela Leland ◽  
Alan L. Schwartz
Keyword(s):  
Cell Line ◽  
Plasmodium Berghei ◽  
Hepatoma Cell ◽  
In Vitro Cultivation ◽  
Hepatoma Cell Line

In vitro cultivation of the exoerythrocytic stage of Plasmodium berghei from sporozoites

Science ◽  
1981 ◽  
Vol 213 (4511) ◽  
pp. 1021-1022 ◽  
Author(s):  
M. Hollingdale ◽  
J. Leef ◽  
M McCullough ◽  
R. Beaudoin
Keyword(s):  
Plasmodium Berghei ◽  
In Vitro Cultivation

The Influence of Cell Type and Culture Medium on the In vitro Cultivation of Exoerythrocytic Stages of Plasmodium berghei

1983 ◽  
Vol 69 (2) ◽  
pp. 346 ◽  
Author(s):  
Michael R. Hollingdale ◽  
Pamela Leland ◽  
James L. Leef ◽  
Richard L. Beaudoin
Keyword(s):  
Plasmodium Berghei ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Cell Type
Sign in / Sign up

Export Citation Format

Share Document