Arterial connective tissue changes and distribution of 125-labelled low density lipoprotein in hypertensive pigs

1980 ◽  
Vol 35 (4) ◽  
pp. 393-411 ◽  
Author(s):  
D.N. Sharpe ◽  
P.J. Scott ◽  
M.H. Flint ◽  
J. Donald
Keyword(s):  
Connective Tissue ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Tissue Changes

scholarly journals Identification of macrophages in sections of rabbit lung using acetoacetylated lipoproteins.

1983 ◽  
Vol 31 (9) ◽  
pp. 1136-1138 ◽  
Author(s):  
A J Hass ◽  
H R Davis ◽  
V M Elner ◽  
S Glagov
Keyword(s):  
Connective Tissue ◽  
Alveolar Macrophages ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Mononuclear Phagocytes ◽  
Low Density ◽  
Modified Technique ◽  
Rabbit Lung ◽  
Tissue Sections ◽  
Thin Slices

Macrophages were labeled in sections of rabbit lung with acetoacetylated low density lipoprotein (LDL), a marker internalized by cultured macrophages but not by other connective tissue cells. Using a modified technique, thin slices of fresh rabbit lung were incubated in 3,3'-dioctadecylindocarbocyanine (DiI)-labeled, acetoacetylated LDL, fixed in paraformaldehyde, and sectioned. Alveolar macrophages incorporated the fluorescently labeled, modified LDL, but surrounding stroma and parenchyma did not stain. Our results indicate that DiI-labeled, acetoacetylated LDL may be used to identify mononuclear phagocytes in tissue sections.

Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

1985 ◽  
Vol 43 ◽  
pp. 546-547 ◽  
Author(s):  
Dean A. Handley ◽  
Cynthia M. Arbeeny ◽  
Larry D. Witte
Keyword(s):  
Colloidal Gold ◽  
Cultured Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coated Vesicle ◽  
Low Density ◽  
Cholesterol Esters ◽  
Cultured Fibroblasts ◽  
Surface Receptors ◽  
Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

Sign in / Sign up

Export Citation Format

Share Document