Immunohistochemical identification of T- and B-lymphocytes delineated by the unlabeled antibody enzyme method. I. Anatomical distribution of Ω-positive and Ig-positive cells in lymphoid organs of mice

1976 ◽  
Vol 13 (3-4) ◽  
pp. 261-270 ◽  
Author(s):  
G. Hoffmann-Fezer ◽  
H. Rodt ◽  
M. Eulitz ◽  
S. Thierfelder
Keyword(s):  
B Lymphocytes ◽  
Lymphoid Organs ◽  
T And B Lymphocytes ◽  
Anatomical Distribution ◽  
Enzyme Method ◽  
Unlabeled Antibody ◽  
Immunohistochemical Identification

ENGRAFTED HUMAN T AND B LYMPHOCYTES FORM MIXED FOLLICLES IN LYMPHOID ORGANS OF HUMAN/MOUSE AND HUMAN/RAT RADIATION CHIMERA1

1997 ◽  
Vol 63 (8) ◽  
pp. 1166-1171 ◽  
Author(s):  
Tanya Burakova ◽  
Hadar Marcus ◽  
Allon Canaan ◽  
Benjamin Dekel ◽  
Elias Shezen ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Lymphoid Organs ◽  
T And B Lymphocytes

Ultrastructural localization of neuropeptides in the rat brain

1978 ◽  
Vol 36 (2) ◽  
pp. 612-613
Author(s):  
F.W. Van Leeuwen
Keyword(s):  
Freeze Substitution ◽  
Ultrastructural Localization ◽  
Serial Sections ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Enzyme Method ◽  
Perfusion Fixation ◽  
Electron Microscopical ◽  
Unlabeled Antibody ◽  
Peroxidase Conjugate

In order to obtain specific and optimal ultrastructural localization of vasopressin and oxytocin in the hypothalamo-neurohypophyseal system of the rat, 2 staining procedures and several tissue treatments were evaluated using neurohypophyseal tissue. It appeared from these studies that post-embedding staining with the unlabeled antibody enzyme method developed by Sternberger allows greater dilution of the first antibody (anti-vasopressin, 1:4800) than the indirect procedure (1:320) using a peroxidase conjugate as second antibody. Immersion fixation with 4% formalin during 24 hours gave better results (general ultrastructure, immunoreactivity) than those obtained by perfusion fixation with 2.5% glutaraldehyde-1% paraformaldehyde or freeze substitution.Since no reliable specificity tests were performed at the electron microscopical level, tests were developed for antibodies against both vasopressin and oxytocin. For anti-vasopressin plasma neural lobes of homozygous Brattleboro rats, that are lacking vasopressin by a genet- ical defect, were used. For antibodies against both hormones serial sections were used that were alternately incubated with the antibodies.

Relationship among T- and B-lymphocytes of the peripheral blood and thyroid tissue in patients with Graves' disease

2019 ◽  
Author(s):  
Margarita A Dudina ◽  
Andrey A Savchenko ◽  
Sergey A Dogadin ◽  
Alexandr G Borisov ◽  
Igor V Kudryavcev ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Peripheral Blood ◽  
Thyroid Tissue ◽  
T And B Lymphocytes ◽  
Graves Disease
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