Enzyme-linked immunosorbent assay for IgG antibodies to Epstein-Barr virus-associated early antigens and viral capsid antigen

Infections can act as environmental triggers that induce or promote systemic lupus erythematosus (SLE) in genetically predisposed individuals. New technologies, developed recently, enable simultaneous assessment of multiple antibodies. Antibodies to specific infectious agents may shed light into the mechanisms of induction of SLE. The aim of this study was to investigate the prevalence of seropositivity and the titers of antibodies to bacterial, viral, and parasitic agents in SLE patients compared with non-autoimmune controls. Sera from 260 individuals (120 SLE patients and 140 controls) were tested by the BioPlex 2200 Multiplexed Immunoassay method (BioRad) for the prevalence and titers of antibodies to eight infectious agents (Epstein—Barr virus: early antigen IgG, nuclear antigen IgG, viral capsid antigen IgG and IgM, heterophile IgM; cytomegalovirus IgG and IgM; Toxoplasma gondii IgG and IgM; rubella IgG and IgM; Treponema pallidum TPr15G, TPr17G, TPr47G; herpes simplex virus type 1 and 2 IgG; hepatitis C virus and hepatitis B core antibodies. Cytomegalovirus IgM and Epstein—Barr virus early antigen IgG (but not other Epstein—Barr virus antigens) were significantly more prevalent in SLE patients than in controls. Conversely, positive titers of hepatitis B core and rubella IgG antibodies were less prevalent in the SLE patients than in controls. Other differences in titer positivity prevalence were not detected between patients and controls. The titers of the cytomegalovirus IgM, Toxoplasma IgG, Epstein—Barr virus early antigen, and viral capsid antigen IgG antibodies were significantly higher in SLE compared with controls. Our data suggest the importance of previous exposure to infectious agents in the induction and the prevention of SLE. Lupus (2009) 18, 1129—1135.

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Cloning of the Rhesus Lymphocryptovirus Viral Capsid Antigen and Epstein-Barr Virus-Encoded Small RNA Homologues and Use in Diagnosis of Acute and Persistent Infections

Journal of Clinical Microbiology ◽

10.1128/jcm.38.9.3219-3225.2000 ◽

2000 ◽

Vol 38 (9) ◽

pp. 3219-3225 ◽

Cited By ~ 35

Author(s):

Pasupuleti Rao ◽

Hua Jiang ◽

Fred Wang

Keyword(s):

Small Rna ◽

Epstein Barr Virus ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

Viral Capsid ◽

Viral Capsid Antigen ◽

Rt Pcr ◽

Barr Virus ◽

Peptide Elisa ◽

Epstein Barr

Epstein-Barr virus (EBV) is the most common cause of infectious mononucleosis and is associated with the development of several human malignancies. A closely related herpesvirus in the same lymphocryptovirus (LCV) genera as EBV naturally infects rhesus monkeys and provides an important animal model for studying EBV pathogenesis. We cloned the small viral capsid antigen (sVCA) homologue from the rhesus LCV and developed a peptide enzyme-linked immunosorbent assay (ELISA) to determine whether epitopes in the rhesus LCV sVCA are a reliable indicator of rhesus LCV infection. In order to define a “gold standard” for rhesus LCV infection, we also cloned the EBV-encoded small RNA 1 (EBER1) and EBER2 homologues from rhesus LCV and developed a reverse transcription (RT)-PCR assay to detect persistent LCV infection in rhesus monkey peripheral blood lymphocytes. Animals from a conventional and a hand-reared colony were studied to compare the prevalence of rhesus LCV infection in the two groups. There was a 100% correlation between the peptide ELISA and EBER RT-PCR results for rhesus LCV infection. In addition, specificity for LCV infection and exclusion of potential cross-reactivity to the rhesus rhadinovirus sVCA homologue could be demonstrated using sera from experimentally infected animals. These studies establish two novel assays for reliable diagnosis of acute and persistent rhesus LCV infections. The rhesus LCV sVCA peptide ELISA provides a sensitive and reliable assay for routine screening, and these studies of the hand-reared colony confirm the feasibility of raising rhesus LCV-naive animals.

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Enzyme-Linked immunosorbent assay (ELISA) for IgA and IgG antibodies to epstein-barr-virus ribonucleotide reductase in patients with nasopharyngeal carcinoma

International Journal of Cancer ◽

10.1002/ijc.2910590604 ◽

1994 ◽

Vol 59 (6) ◽

pp. 739-742 ◽

Cited By ~ 11

Author(s):

A. Fones-Tan ◽

S. H. Chan ◽

S. Y. Tsao ◽

L. H. Gan ◽

W. H. Tan ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Immunosorbent Assay ◽

Ribonucleotide Reductase ◽

Epstein Barr Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Igg Antibodies ◽

Barr Virus ◽

Epstein Barr

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Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using a Synthetic Convergent Peptide Library, or Mixotope, for Diagnosis of Primary EBV Infection

Journal of Clinical Microbiology ◽

10.1128/jcm.37.7.2366-2368.1999 ◽

1999 ◽

Vol 37 (7) ◽

pp. 2366-2368 ◽

Cited By ~ 7

Author(s):

D. Tranchand-Bunel ◽

H. Gras-Masse ◽

B. Bourez ◽

L. Dedecker ◽

C. Auriault

Keyword(s):

Immunosorbent Assay ◽

Epstein Barr Virus ◽

Peptide Library ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Viral Capsid Antigen ◽

Amino Acid Peptide ◽

Barr Virus ◽

Ebv Infection ◽

Epstein Barr

An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.

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