Phosphorylation of cardiac native tropomyosin and troponin: Inhibitory effect of actomyosin and possible presence of endogenous myofibrillar-located cyclic-AMP-dependent protein kinase

1973 ◽  
Vol 5 (5) ◽  
pp. 461-464 ◽  
Author(s):  
Y Reddy
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals Association of cyclic-AMP-dependent protein kinase with neurofilaments

1992 ◽  
Vol 282 (2) ◽  
pp. 477-481 ◽  
Author(s):  
A Dosemeci ◽  
H C Pant
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Kinase Activity ◽  
Inhibitory Effect ◽  
Catalytic Subunit ◽  
Regulatory Subunit ◽  
Dependent Manner ◽  
Dependent Protein Kinase ◽  
Bovine Heart ◽  
Dependent Protein

Neurofilament preparations isolated from bovine spinal cord contain cyclic-AMP-dependent protein kinase (PKA) activity. Treatment of this preparation with cyclic AMP, to dissociate the regulatory subunit of the kinase from the catalytic subunit, resulted in retention of the kinase activity but loss of cyclic AMP regulation. This suggests that PKA is associated via its catalytic subunit with the neurofilament preparation. The association of exogenous PKA from bovine heart with the neurofilament preparation and with neurofilaments reconstituted from purified neurofilament proteins was also investigated. Either the free catalytic subunit or combinations of the catalytic and regulatory subunits of PKA were incubated with the preparations, and the degree of association was determined as the level of kinase activity that co-sediments with neurofilaments. The results indicate that the free catalytic subunit of PKA co-sediments with neurofilaments reconstituted from purified proteins. The regulatory subunit of PKA from bovine heart, when pre-mixed with the catalytic subunit, decreased the level of kinase that co-sediments with the neurofilament fraction in a dose-dependent manner. This effect of the regulatory subunit was reversed by inclusion of cyclic AMP in the incubation medium before centrifugation. The above findings suggest that the regulatory subunit, when attached to the catalytic subunit, has an inhibitory effect on its association with neurofilaments, with the implication that the association may be a cyclic-AMP-regulated event.

scholarly journals A cytosolic cyclic AMP-dependent protein kinase in Dictyostelium discoideum. I. Properties.

1984 ◽  
Vol 259 (1) ◽  
pp. 654-661 ◽  
Author(s):  
I H Majerfeld ◽  
B H Leichtling ◽  
J A Meligeni ◽  
E Spitz ◽  
H V Rickenberg
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Dependent Protein Kinase ◽  
Dependent Protein
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