Ca2+ activation properties of myosin light chain kinase in neurally stimulated tracheal smooth muscle

1992 ◽  
Vol 24 ◽  
pp. 241
Author(s):  
Kristine E. Kamm ◽  
R. Ann Word ◽  
James T. Stull
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Tracheal Smooth Muscle

GTP gamma S-dependent regulation of smooth muscle contractile elements

1992 ◽  
Vol 262 (2) ◽  
pp. C405-C410 ◽  
Author(s):  
Y. Kubota ◽  
M. Nomura ◽  
K. E. Kamm ◽  
M. C. Mumby ◽  
J. T. Stull
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Protein Phosphatase ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Kinase Activity ◽  
Tracheal Smooth Muscle ◽  
Proportional Increase ◽  
Gamma S ◽  
Muscle Contractile

Guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) increases the sensitivity of the contractile response to activation by Ca2+ in permeabilized tracheal smooth muscle. Increased tension was associated with a proportional increase in myosin light chain phosphorylation. The site of phosphorylation was determined to be serine-19, which corresponds to the site rapidly phosphorylated by myosin light chain kinase. GTP gamma S did not affect the contraction induced by the protein phosphatase inhibitor okadaic acid but did enhance contraction produced by Ca(2+)-independent myosin light chain kinase. In tracheal homogenates Ca(2+)-dependent myosin light chain kinase activity was not affected by GTP gamma S; however, dephosphorylation of 32P-labeled heavy meromyosin by phosphatase was inhibited. Thus GTP gamma S may increase the Ca2+ sensitivity of contractile elements in tracheal smooth muscle by inhibition of protein phosphatase activity toward myosin light chain.

scholarly journals Ontogenesis of myosin light chain kinase mRNA and protein content in guinea pig tracheal smooth muscle

2004 ◽  
Vol 38 (6) ◽  
pp. 456-464 ◽  
Author(s):  
Pasquale Chitano ◽  
Judith A. Voynow ◽  
Valeria Pozzato ◽  
Viviana Cantillana ◽  
Lauranell H. Burch ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Protein Content ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Tracheal Smooth Muscle
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