Construction and characterization of a plasmid containing a nearly full-size DNA copy of satellite tobacco necrosis virus RNA

1980 ◽  
Vol 143 (3) ◽  
pp. 259-271 ◽  
Author(s):  
John van Emmelo ◽  
René Devos ◽  
Mark Ysebaert ◽  
Walter Fiers
Keyword(s):  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Full Size ◽  
Virus Rna

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

Molecular characterization of a Hungarian isolate of Tobacco necrosis virus A

2010 ◽  
Vol 155 (6) ◽  
pp. 999-1001
Author(s):  
Laszlo Krizbai ◽  
Maria Nemeth ◽  
Vivian Law ◽  
Christopher Reed ◽  
Aniko Varga ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation products

Biochemistry ◽  
1972 ◽  
Vol 11 (11) ◽  
pp. 2009-2014 ◽  
Author(s):  
William H. Klein ◽  
Chris Nolan ◽  
Jerome M. Lazar ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus
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