scholarly journals Evidence that the requirements for ATP and wheat germ initiation factors 4A and 4F are affected by a region of satellite tobacco necrosis virus RNA that is 3' to the ribosomal binding site.

1988 ◽  
Vol 263 (17) ◽  
pp. 8380-8383 ◽  
Author(s):  
K S Browning ◽  
L Fletcher ◽  
J M Ravel
Keyword(s):  
Binding Site ◽  
Wheat Germ ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Initiation Factors ◽  
Virus Rna ◽  
Ribosomal Binding Site

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

Translation of satellite tobacco necrosis virus ribonucleic acid by an in vitro system from wheat germ

Biochemistry ◽  
1976 ◽  
Vol 15 (22) ◽  
pp. 4943-4950 ◽  
Author(s):  
David W. Leung ◽  
Carl W. Gilbert ◽  
Robert E. Smith ◽  
Nancy L. Sasavage ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
Wheat Germ ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
In Vitro System

Two 3′-Terminal Sequences in Satellite Tobacco Necrosis Virus RNA

Nature ◽  
1969 ◽  
Vol 221 (5186) ◽  
pp. 1122-1126 ◽  
Author(s):  
E. WIMMER ◽  
M. E. REICHMANN
Keyword(s):  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Virus Rna
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