scholarly journals The 5' and 3' untranslated regions of satellite tobacco necrosis virus RNA affect translational efficiency and dependence on a 5' cap structure

1993 ◽  
Vol 268 (13) ◽  
pp. 9504-9510
Author(s):  
R.T. Timmer ◽  
L.A. Benkowski ◽  
D. Schodin ◽  
S.R. Lax ◽  
A.M. Metz ◽  
...  
Keyword(s):  
Untranslated Regions ◽  
Tobacco Necrosis Virus ◽  
Translational Efficiency ◽  
Necrosis Virus ◽  
Virus Rna

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

Two 3′-Terminal Sequences in Satellite Tobacco Necrosis Virus RNA

Nature ◽  
1969 ◽  
Vol 221 (5186) ◽  
pp. 1122-1126 ◽  
Author(s):  
E. WIMMER ◽  
M. E. REICHMANN
Keyword(s):  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Virus Rna

Sequence studies of satellite tobacco necrosis virus RNA

1968 ◽  
Vol 38 (1) ◽  
pp. 59-73 ◽  
Author(s):  
Eckard Wimmer ◽  
Albert Y. Chang ◽  
J.M. Clark ◽  
M.E. Reichmann
Keyword(s):  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Virus Rna
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