Growth inhibitory effect of somatostatin (SS) on human breast cancer cells in culture

1987 ◽  
Vol 28 ◽  
pp. 165 ◽  
Author(s):  
G. Scambia ◽  
P. Benedetti Panici ◽  
G. Baiocchi ◽  
C. Andreani ◽  
C. Gaggini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Inhibitory Effect ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Growth Inhibitory Effect ◽  
Cells In Culture ◽  
Growth Inhibitory

Kinetics of cyclocreatine and Na+ cotransport in human breast cancer cells: mechanism of activity

1999 ◽  
Vol 277 (4) ◽  
pp. C708-C716 ◽  
Author(s):  
Nimrod Maril ◽  
Hadassa Degani ◽  
Edna Rushkin ◽  
A. Dean Sherry ◽  
Mildred Cohn
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Inhibitory Effect ◽  
Human Breast Cancer Cells ◽  
Resistant Line ◽  
Human Breast ◽  
Growth Inhibitory Effect ◽  
Growth Inhibitory ◽  
Kinetics Of

The growth-inhibitory effect of cyclocreatine (CCr) and the kinetics of CCr and Na+ cotransport were investigated in MCF7 human breast cancer cells and its adriamycin-resistant subline with use of 31P- and23Na-NMR spectroscopy. The growth-inhibitory effect in the resistant line occurred at a lower CCr concentration and was more pronounced than in the wild-type line. This correlated with an ∼10-fold higher affinity of CCr to the transporter in the resistant line. The passive diffusion coefficient of CCr was also higher in the resistant line by three- to fourfold. The transport of CCr was accompanied by a rapid increase in intracellular Na+. This increase was found to depend on the rate of CCr transport and varied differently with CCr concentration in the two cell lines. It is proposed that the cotransport of CCr and Na+followed by increased Na+concentration, together with the accumulation of the highly charged phosphocyclocreatine, are responsible for cell swelling and death.

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