III. Determination of plasma catecholamines and free 3, 4-dihydroxyphenylacetic acid in continuously collected human plasma by high performance liquid chromatography with electrochemical detection

Life Sciences ◽  
1981 ◽  
Vol 28 (5) ◽  
pp. 477-483 ◽  
Author(s):  
Ivan N. Mefford ◽  
Marcia M. Ward ◽  
Laughton Miles ◽  
Barr Taylor ◽  
Margaret A. Chesney ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Electrochemical Detection ◽  
High Performance ◽  
Plasma Catecholamines ◽  
Dihydroxyphenylacetic Acid

Simplified determination of serotonin in plasma by liquid chromatography with electrochemical detection.

1984 ◽  
Vol 30 (1) ◽  
pp. 131-135 ◽  
Author(s):  
P C Tagari ◽  
D J Boullin ◽  
C L Davies
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Electrochemical Detection ◽  
High Performance ◽  
Carcinoid Syndrome ◽  
Quantitative Detection ◽  
Chromatographic Purification ◽  
Hydroxyindoleacetic Acid

Abstract This simplified determination of serotonin in human plasma involves "high-performance" liquid chromatography with electrochemical detection. Deproteinated samples of platelet-poor plasma are injected directly into the chromatograph without preliminary chromatographic purification. The limit of quantitative detection of serotonin in plasma is 5 nmol/L (equivalent to 250 fmol injected). Intra- and inter-assay CVs ranged from 5.6% to 7.7% and 3.5% to 14.9%, respectively, for concentrations of serotonin from 0.02 to 10.0 mumol/L. Results agreed well with those by spectrofluorometry, both for plasma standards (r = 0.985, range 100 nmol/L to 100 mumol/L) and for pathological samples obtained from four carcinoid syndrome patients (r = 0.964). The method is also applicable to the concurrent measurement of 5-hydroxytryptophan and 5-hydroxyindoleacetic acid.

Sign in / Sign up

Export Citation Format

Share Document