Rapid, concurrent analysis of dopamine, 5-hydroxytryptamine, their precursors and metabolites utilizing high performance liquid chromatography with electrochemical detection: Analysis of brain tissue and cerebrospinal fluid

Life Sciences ◽  
1982 ◽  
Vol 31 (25) ◽  
pp. 2847-2856 ◽  
Author(s):  
J.A. Nielsen ◽  
C.A. Johnston
Keyword(s):  
Cerebrospinal Fluid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Brain Tissue ◽  
Electrochemical Detection ◽  
High Performance ◽  
Detection Analysis ◽  
Concurrent Analysis

scholarly journals Distribution of Dopamine in 35 Subregions of the Rat Caudate-Putamen: A High Performance Liquid Chromatography with Electrochemical Detection Analysis

1982 ◽  
Vol 9 (4) ◽  
pp. 421-427 ◽  
Author(s):  
Thérèse Di Paolo ◽  
Michel Daigle ◽  
André Dupont
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Major Axis ◽  
Tissue Preparation ◽  
Caudate Putamen ◽  
Tissue Samples ◽  
Analytical Technique ◽  
Detection Analysis

SUMMARY:Topographical variations in the endogenous content of dopamine were studied in the rat caudate-putamen. For this purpose, a high performance liquid chromatography with electrochemical detection procedure for the determination of norepinephrine, epinephrine and dopamine in nervous tissue samples is described. Tissue preparation required only homogenization in acidic solution and centrifugation prior to application to the chromatograph. Detection limits in the low picogram range were found for the catecholamines separated. This assay was used in combination with a micropunch dissection technique. The performance of this analytical technique is illustrated by the detailed mapping of dopamine content in 35 subregions of the rat caudate-putamen. The distribution of dopamine varies along the major axis of the nucleus.

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