On the occurrence of mucous cells in papillary cystadenoma lymphomatosum

1957 ◽  
Vol 10 (10) ◽  
pp. 1091-1093 ◽  
Author(s):  
Robert J. Gorlin ◽  
A.P. Chaudhry
Author(s):  
Iracema M. Baccarini

Some morphological nuclear features (invaginations) in normal and abnormal cells have been described in several electron microscopic studies. They have been referred to by others as blebs, loops, pockets, sheets, bodies, nuclear inclusions and cytoplasmic invaginations. Identical appearing structures were found in cells of the uterine cervical epithelium, in trophoblasts of blastocysts and in trophoblasts of rat placenta.Methods. Uterine cervix (normal rats), rat placenta (9-10 days gestation) and blastocyst were placed in 3% glutarahdehyde for 3 hours. The tissue was washed in phosphate buffer for 24 hours, postfixed in 1%. buffered osmium tetroxide for 1-2 hours and embedded in epon araldite. Sections were double stained with uranyl acetate and lead citrate and viewed in E. M. Siemens 200.Observations. Nuclear invaginations were found in basal, parabasal and mucous cells of the cervix epithelium, in trophoblasts of blastocyst and in trophoblasts of placenta. An oval, round or elongated invagination contained heterogenously cytoplasm surrounded by a double intact membrane; usually several invaginations were found in the same nucleus.


2001 ◽  
Vol 120 (5) ◽  
pp. A708-A709
Author(s):  
T KANEKO ◽  
H OTA ◽  
M HAYAMA ◽  
K NAKAJIMA ◽  
A YOSHIZAWA ◽  
...  

1970 ◽  
Vol 138 (3) ◽  
pp. 286-305 ◽  
Author(s):  
NICHOLAS D. HOLLAND ◽  
MICHAEL T. GHISELIN

2003 ◽  
Vol 51 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Marco Piludu ◽  
Sean A. Rayment ◽  
Bing Liu ◽  
Gwynneth D. Offner ◽  
Frank G. Oppenheim ◽  
...  

The human salivary mucins MG1 and MG2 are well characterized biochemically and functionally. However, there is disagreement regarding their cellular and glandular sources. The aim of this study was to define the localization and distribution of these two mucins in human salivary glands using a postembedding immunogold labeling method. Normal salivary glands obtained at surgery were fixed in 3% paraformaldehyde-0.1% glutaraldehyde and embedded in Lowicryl K4M or LR Gold resin. Thin sections were labeled with rabbit antibodies to MG1 or to an N-terminal synthetic peptide of MG2, followed by gold-labeled goat anti-rabbit IgG. The granules of all mucous cells of the submandibular and sublingual glands were intensely reactive with anti-MG1. No reaction was detected in serous cells. With anti-MG2, the granules of both mucous and serous cells showed reactivity. The labeling was variable in both cell types, with mucous cells exhibiting a stronger reaction in some glands and serous cells in others. In serous granules, the electron-lucent regions were more reactive than the dense cores. Intercalated duct cells near the acini displayed both MG1 and MG2 reactivity in their apical granules. In addition, the basal and lateral membranes of intercalated duct cells were labeled with anti-MG2. These results confirm those of earlier studies on MG1 localization in mucous cells and suggest that MG2 is produced by both mucous and serous cells. They also indicate differences in protein expression patterns among salivary serous cells.


Life Sciences ◽  
1998 ◽  
Vol 62 (16) ◽  
pp. PL259-PL264 ◽  
Author(s):  
Takafumi Ichikawa ◽  
Kazuhiko Ishihara ◽  
Katsunori Saigenji ◽  
Kyoko Hotta

Development ◽  
1973 ◽  
Vol 29 (1) ◽  
pp. 221-237
Author(s):  
J. Geraudie ◽  
Y. François

The first stages of genesis of the pelvic fin Anlage in the Trout (Salmo fario and S. gairdneri). I. Anatomical study The Anlage of the pelvic fins appears in Salmo 2 weeks after the fecundation day, at the level of the somites 23–26. The mesoderm has a double origin and seems to differ in this regard from the hind limb of most of the amniotes. The ‘initial mesenchyme’ comes from the local proliferation of the somatopleura. It will give essentially the skeletal components of dermal origin (actinotrichia and lepidotrichia). The ‘secondary mesenchyme’ is obtained by the dispersion of four ventral somitic processes that have reached and entered the initial mesenchyme blastema. The secondary mesenchyme will probably give the muscles and also the endoskeleton of the fin. The origin of the girdle is not clear. When the setting of the initial mesenchyme begins the epithelium that covers the embryo is already differentiated in an epiderm with numerous mucous cells resting on a visible basement membrane. At the apex of the pelvic bud, a localized and transitory thickening of the epiderm is produced by the increase in the height of the basal stratum. We call this structure by the name of ‘pseudo apical cap’ to stress the fact that it must be distinguished from the ‘apical cap’ described for the limb bud of amniotes. So, the morphogenesis of the pelvic fins of Salmo shows some important particularities in the epiderm as well as in the mesoderm.


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