A serological survey of antibodies to Streptomyces somaliensis and Actinomadura madurae in the Sudan using enzyme linked immunosorbent assay (ELISA)

ABSTRACT: Bluetongue is an infectious, non-contagious disease that affects domestic and wild ruminants, caused by a virus from the Orbivirus genus, Reoviridae family, transmitted by arthropod vectors of the Culicoides genus. This paper aims to be the first serological survey of bluetongue in sheep from the Meso-regions of Campo das Vertentes and South and Southeast of Minas Gerais. Samples were collected from sheep from different properties. The serum samples were submitted to Agar Gel Immunodiffusion (AGID) and competitive Enzyme-Linked Immunosorbent Assay (cELISA). 303 serum samples were submitted to AGID and cELISA. In these samples, 164 (54.13%) were positive in the AGID technique, and 171 (56.44%) positive in the cELISA technique, with an almost perfect agreement between the techniques (kappa index = 0.887). In all visited properties, positive animals have been found in the herd. Animals acquired from properties of the studied mesoregions were more likely to be positive in IDGA and cELISA tests than animals acquired from properties in other regions of Brazil (p<0.001). These results suggest that bluetongue virus (BTV) is widespread in the mesoregions of Campo das Vertentes and South and Southeast of Minas Gerais.

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Development and application of an indirect enzyme-linked immunosorbent assay for serological survey of Japanese encephalitis virus infection in dogs

Journal of Virological Methods ◽

10.1016/j.jviromet.2012.09.022 ◽

2013 ◽

Vol 187 (1) ◽

pp. 85-89 ◽

Cited By ~ 7

Author(s):

Hiroshi Shimoda ◽

Natnaree Inthong ◽

Keita Noguchi ◽

Yutaka Terada ◽

Yumiko Nagao ◽

...

Keyword(s):

Virus Infection ◽

Japanese Encephalitis Virus ◽

Japanese Encephalitis ◽

Immunosorbent Assay ◽

Encephalitis Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Serological Survey ◽

Japanese Encephalitis Virus Infection

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Serological survey of parapoxvirus infection in wild ruminants in Japan in 1996–9

Epidemiology and Infection ◽

10.1017/s0950268801005131 ◽

2001 ◽

Vol 126 (1) ◽

pp. 153-156 ◽

Cited By ~ 14

Author(s):

Y. INOSHIMA ◽

Y. YAMAMOTO ◽

T. TAKAHASHI ◽

M. SHINO ◽

A. KATSUMI ◽

...

Keyword(s):

Immunosorbent Assay ◽

Cervus Nippon ◽

Enzyme Linked Immunosorbent Assay ◽

Serological Survey ◽

Serum Samples ◽

Agar Gel ◽

Wild Ruminants ◽

Immunodiffusion Test ◽

Japanese Serow ◽

Free Ranging

The prevalence of parapoxvirus infection was examined in free-ranging wild ruminants in Japan, Japanese serow (Capricornis crispus) and Japanese deer (Cervus nippon centralis), in 1996–9. We collected a total of 151 serum samples from 101 Japanese serows and 50 Japanese deer and tested for antibodies against parapoxvirus by an enzyme-linked immunosorbent assay and an agar gel immunodiffusion test. Overall seroprevalences among Japanese serows were 5/25 (20·0%) in 1996, 4/14 (28·6%) in 1997, 5/32 (15·6%) in 1998 and 2/30 (6·7%) in 1999, respectively. The seroprevalence increased with age but was not affected by sex. No antibodies were detected from any of 50 serum samples taken from Japanese deer. Our results in this study suggest that parapoxvirus infection is widespread among the population of Japanese serows, however, Japanese deer appear to be still free of the disease.

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