Long-Term Determinants of the Seroprevalence of the Hepatitis E Virus in Wild Boar (Sus scrofa)

The hepatitis E virus (HEV) is an emerging zoonotic pathogen whose main reservoir is suids. Most of the ecological and epidemiological aspects of its sylvatic cycle remain unknown. Thus, in this work, we study the drivers of HEV exposure in the wild boar population of Doñana National Park (DNP, southwest Spain) operating in the medium and long-term (2005–2018). Anti-HEV antibodies are widely distributed throughout the wild boar (46.7 ± 3.8%, 327 out of 700 sampled), showing a statistically significant age-increasing pattern. The temporal pattern displayed important interannual fluctuations. This could be mediated by marked variations in the population control of the wild boar, and subsequent changes in abundance rates, and its interplay with climatic conditions; as wet years together with a low abundance of wild boar led to the lowest seroprevalence. The fact that seroprevalence is high during conditions of high abundance, and not affected by rainfall level, is probably due to the increased interactions among the animals, and possibly, the subsequent higher environmental contamination with HEV particles. The proximity to the marshland (the main water body of the study area) is associated with a higher risk of testing positive, which is probably mediated by the preferential use of this area during the dry season and the favourable environmental conditions for the survival of HEV particles. A deeper understanding of the epidemiology of HEV in host communities deserves future research concerning other susceptible species. Most importantly, wild boar population control remains a challenge at the international level, and an increase of shared pathogen-related conflicts associated with this species is expected, as exemplified by HEV. Therefore, surveillance of wild boar diseases, including integrated population monitoring and sustainable population control programmes, will be essential to control the associated risks.

The dark-ventral-patch of male red deer, a sexual signal that conveys the degree of involvement in rutting behavior

Background In polygynous mammals, signalling may play a decisive role in mating behavior, mediating the intensity of male fights and female mate choice. During the rutting season, male red deer may show a visible dark patch in their ventral fur. Recently, this patch has been suggested to act as a flexible sexual signal, due to its relationships with other variables such as age, body size, antler development, volatile compounds, or the competitive environment. The analysis of fur pigmentation at the ventral patch suggests that this might also visually indicate the male intrinsic predisposition to take part in mating competition. Results To assess the possible role of this trait as a communicative signal related to mate competition, we used red deer behavioral observations during the rut in Doñana National Park (Spain) to examine the link between the degree of expression of the dark ventral patch and the rutting activity (assessed from both intra-and-inter-sexual behaviors). Consistent with our predictions, we found in a field study that males with large dark patches showed a higher frequency of rutting behaviors (mainly roaring and flehmen), more interactions with females, and attained larger harem sizes. Conclusions The dark ventral patch was a better predictor of male behavior than antler tines or territory holding, thus standing as a short-term indicator of male willingness to invest in mating competition.

Improvement of Strawberry Irrigation Sustainability in Southern Spain Using FAO Methodology

Irrigation sustainability is particularly important in the vicinity of Doñana National Park (Huelva, Spain), where Europe’s most important wetland area coexists with a profitable strawberry irrigation activity. In this paper, an innovation and technology transfer project was laid out. The project was promoted by the Institute of Agricultural and Fisheries Research and Training (IFAPA), belonging to the Regional Government of Andalusia. The main objective of the project was to contribute to the sustainability of the complex ecological, productive, and social system of this region. The project was focused on the rational use of water resources. Experimentation, demonstration, technology transfer, and training activities were carried out, involving public administrations, companies, and private farms. The project was carried out in collaboration with strawberry companies covering a total surface area of 1900 hectares. Irrigation application efficiency and irrigation water productivity increased by 66% and there was also a significant increase in water saving (44%), without resulting production losses. The success of the activity was based on the implication of farmers in experimentation assignments. During a five-year time span, irrigation trials took place on several farms. This fact allowed a progressive improvement of irrigation management by farmers based on confidence in the experimental work results.

Environmental Citizenship Education through the Doñana, Biodiversity and Culture Program

While the need to educate for the formation of environmentally committed citizens is something that has always been recognized, currently however it has become an obligation. The present study analyzes the Doñana, Biodiversity and Culture Program from the perspective of its participants. The program is part of the action being carried out with schools intending to teach environmental citizenry using the context of protected natural spaces, in this specific case, the Doñana National Park. The Park’s managers and public guides were interviewed, and observation records of the process were collected. These were later analyzed by means of a category table elaborated within the project Patrimonial Education for Citizens’ Territorial and Emotional Intelligence, of which this study is a part. The consistency of the informants was verified, as also was the practice observed, showing the importance of the socio-identity framework, of critical thinking, and of socio-affective relationships with the territory.

Long-Term Determinants of the Seroprevalence of Toxoplasma gondii in a Wild Ungulate Community

Toxoplasma gondii is an obligate intracellular protozoan which infects warm-blooded vertebrates, including humans, worldwide. In the present study, the epidemiology of T. gondii was studied in the wild ungulate host community (wild boar, red deer, and fallow deer) of Doñana National Park (DNP, south-western Spain) for 13 years (2005–2018). We assessed several variables which potentially operate in the medium and long-term (environmental features, population, and stochastic factors). Overall, the wild ungulate host community of DNP had high seroprevalence values of T. gondii (STG; % ± confidence interval (CI) 95%; wild boar (Sus scrofa) 39 ± 3.3, n = 698; red deer (Cervus elaphus) 30.7 ± 4.4, n = 423; fallow deer (Dama dama) 29.7 ± 4.2, n = 452). The complex interplay of hosts and ecological/epidemiological niches, together with the optimal climatic conditions for the survival of oocysts that converge in this area may favor the spread of the parasite in its host community. The temporal evolution of STG oscillated considerably, mostly in deer species. The relationships shown by statistical models indicated that several factors determined species patterns. Concomitance of effects among species, indicated that relevant drivers of risk operated at the community level. Our focus, addressing factors operating at broad temporal scale, allows showing their impacts on the epidemiology of T. gondii and its trends. This approach is key to understanding the epidemiology and ecology to T. gondii infection in wild host communities in a context where the decline in seroprevalence leads to loss of immunity in humans.

Taphonomic information from the modern vertebrate death assemblage of Doñana National Park, Spain

Modern death assemblages provide insights about the early stages of fossilization and useful ecological information about the species inhabiting the ecosystem. We present the results of taphonomic monitoring of modern vertebrate carcasses and bones from Doñana National Park, a Mediterranean coastal ecosystem in Andalusia, Spain. Ten different habitats were surveyed. Half of them occur in active depositional environments (marshland, lake margin, river margin, beach and dunes). Most of the skeletal remains belong to land mammals larger than 5 kg in body weight (mainly wild and feral ungulates). Overall, the Doñana bone assemblage shows good preservation with little damage to the bones, partly as a consequence of the low predator pressure on large vertebrates. Assemblages from active depositional habitats differ significantly from other habitats in terms of the higher incidence of breakage and chewing marks on bones in the latter, which result from scavenging, mainly by wild boar and red fox. The lake-margin and river-margin death assemblages have high concentrations of well preserved bones that are undergoing burial and offer the greatest potential to produce fossil assemblages. The spatial distribution of species in the Doñana death assemblage generally reflects the preferred habitats of the species in life. Meadows seem to be a preferred winter habitat for male deer, given the high number of shed antlers recorded there. This study is further proof that taphonomy can provide powerful insights to better understand the ecology of modern species and to infer past and future scenarios for the fossil record.