Elasmobranch tissue culture: In vitro growth of brain explants from a shark (Rhizoprionodon) and dogfish (Squalus)

1988 ◽  
Vol 20 (5) ◽  
pp. 759-761 ◽  
Author(s):  
William Dean A. Garner

1962 ◽  
Vol 26 (1) ◽  
pp. 129-135 ◽  
Author(s):  
J. Michl


Development ◽  
1964 ◽  
Vol 12 (4) ◽  
pp. 575-585
Author(s):  
Andrzej K. Tarkowski

A General description of the development of mouse chimaerae and an account of the techniques for their production were given in previous reports (Tarkowski, 1961, 1963). The chimaeric character of the embryos and young obtained was tentatively claimed in the first of these publications because (1) the actual union of two eggs into one blastocyst was seen in culture in vitro, (2) of the occurrence of intersexes, (3) pigment synthesis of the types of the dark component occurred in the majority of individuals developed from pairs of eggs differing genetically in factors for pigmentation. The last criterion was met only by macroscopic search for pigment in the eyes. The present report gives a more detailed description of the distribution of pigment forming cells in these animals, based on histological analysis. Some remarks on the validity and applicability of such a criterion for estimating the degree of chimaerism were made at the 13th Annual Meeting of the Tissue Culture Association (Tarkowski 1963).



1980 ◽  
Vol 29 (3) ◽  
pp. 940-944 ◽  
Author(s):  
A E Metzler ◽  
R J Higgins ◽  
S Krakowka ◽  
A Koestner

Virulence of canine distemper virus (CDV) adapted to in vitro growth in Vero or bovine cells was determined by inoculation into CDV-susceptible neonatal gnotobiotic dogs. When compared with dogs given virulent R252-CDV, Vero R252-CDV was attenuated at passage level 14. In contrast, dogs inoculated with bovine R252-CDV at the same passage level experienced rapid fatal neurological disease. Virulence was not linked to ability to infect or replicate in canine pulmonary macrophage cultures. Retention of virulence by bovine R252-CDV is unique and worthy of further study.



2017 ◽  
pp. 425-435 ◽  
Author(s):  
Marina Barba ◽  
Munetaka Hosakawa ◽  
Qiao-Chun Wang ◽  
Anna Taglienti ◽  
Zhibo Zhang


Author(s):  
K.E. Bailey ◽  
N.J. Darling ◽  
D. Velu ◽  
T.J. D'Ovidio ◽  
M.L. Floren ◽  
...  






1930 ◽  
Vol 28 (3) ◽  
pp. 282-284 ◽  
Author(s):  
M. Sano ◽  
L. W. Smith


2013 ◽  
Vol 43 (2) ◽  
pp. 138-146 ◽  
Author(s):  
João Paulo Rodrigues Martins ◽  
Edilson Romais Schimildt ◽  
Rodrigo Sobreira Alexandre ◽  
Breno Régis Santos ◽  
Gizele Cristina Magevski

The tissue culture can contribute to the propagation of several economic species, such as the bromeliads. This research aimed at evaluating the auxins type and concentration in the in vitro and ex vitro rhizogenesis of Neoregelia concentrica bromeliad. N. concentrica shoots were induced in a growth medium with 15.0 µM of 6-benzylaminopurine, for 80 days, followed by sub-cultivation in phytoregulator-free medium, for 45 days. In the in vitro rhizogenesis, the shoots grew in a medium supplemented with indole-3-butyric acid (IBA) or naphthalene-acetic acid (NAA), at the concentrations of 0.0 µM, 1.0 µM, 2.0 µM, 3.0 µM and 4.0 µM. In the ex vitro rhizogenesis, the bases of shoots were immersed, for 60 minutes, in IBA or NAA solutions, at the concentrations of 0.0 µM, 5.0 µM, 10.0 µM and 15.0 µM. After immersion, the shoots were planted in plastic trays with vermiculite. At the end of each rhizogenesis method, the phytotechnical parameters analysis was carried out. For the in vitro rhizogenesis, a higher number of roots were observed when the shoots were cultivated in concentrations higher than 1.0 µM of NAA, when compared to the IBA. However, the rooting rate differed only at 30 days after the in vitro growth, with a higher root induction in the shoots grown with NAA. At 60 days, the rooting rate was higher than 90% and statistically similar in all treatments. In the ex vitro rhizogenesis, a better formation of the rooting system was observed when 5.0 µM of IBA was applied, with higher rooting averages and number of roots.



JAMA ◽  
1925 ◽  
Vol 84 (25) ◽  
pp. 1943


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