Virulence of tissue culture-propagated canine distemper virus

1980 ◽  
Vol 29 (3) ◽  
pp. 940-944 ◽  
Author(s):  
A E Metzler ◽  
R J Higgins ◽  
S Krakowka ◽  
A Koestner
Keyword(s):  
Tissue Culture ◽  
Neurological Disease ◽  
Canine Distemper Virus ◽  
In Vitro Growth ◽  
Canine Distemper ◽  
Passage Level ◽  
Pulmonary Macrophage

Virulence of canine distemper virus (CDV) adapted to in vitro growth in Vero or bovine cells was determined by inoculation into CDV-susceptible neonatal gnotobiotic dogs. When compared with dogs given virulent R252-CDV, Vero R252-CDV was attenuated at passage level 14. In contrast, dogs inoculated with bovine R252-CDV at the same passage level experienced rapid fatal neurological disease. Virulence was not linked to ability to infect or replicate in canine pulmonary macrophage cultures. Retention of virulence by bovine R252-CDV is unique and worthy of further study.

scholarly journals Virulent and attenuated canine distemper virus infects multiple dog brain cell types in vitro

Glia ◽  
1991 ◽  
Vol 4 (4) ◽  
pp. 408-416 ◽  
Author(s):  
Susan Pearce-Kelling ◽  
William J. Mitchell ◽  
Brian A. Summers ◽  
Max J. G. Appel
Keyword(s):  
Canine Distemper Virus ◽  
Cell Types ◽  
Brain Cell ◽  
Canine Distemper ◽  
Dog Brain ◽  
Brain Cell Types

scholarly journals Canine distemper virus-induced glial cell changes in vitro

1983 ◽  
Vol 62 (1-2) ◽  
pp. 51-58 ◽  
Author(s):  
A. Zurbriggen ◽  
M. Vandevelde
Keyword(s):  
Glial Cell ◽  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Cell Changes

scholarly journals Complement-Mediated Neutralization of Canine Distemper Virus In Vitro: Cross-Reaction between Vaccine Onderstepoort and Field KDK-1 Strains with Different Hemagglutinin Gene Characteristics

2002 ◽  
Vol 9 (4) ◽  
pp. 921-924 ◽  
Author(s):  
Masami Mochizuki ◽  
Megumi Motoyoshi ◽  
Ken Maeda ◽  
Kazunari Kai
Keyword(s):  
Guinea Pig ◽  
Canine Distemper Virus ◽  
Field Isolate ◽  
Neutralization Assay ◽  
Cross Reaction ◽  
Canine Distemper ◽  
Hemagglutinin Gene

ABSTRACT The properties of neutralization of antigens of canine distemper virus Onderstepoort and a recent field isolate, KDK-1, were investigated with strain-specific dog sera. A conventional neutralization assay indicated antigenic dissimilarity between the strains; however, when guinea pig complement was included in the reaction mixture, the strains were neutralized with not only the homologous but also the heterologous antibodies.

scholarly journals Recovery of NanoLuc Luciferase-Tagged Canine Distemper Virus for Facilitating Rapid Screening of Antivirals in vitro

2020 ◽  
Vol 7 ◽  
Author(s):  
Fuxiao Liu ◽  
Qianqian Wang ◽  
Yilan Huang ◽  
Ning Wang ◽  
Youming Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Canine Distemper Virus ◽  
Reverse Genetics ◽  
Rapid Screening ◽  
Canine Distemper ◽  
Virus Propagation ◽  
The Family ◽  
Conventional Methods

Canine distemper virus (CDV), belonging to the genus Morbillivirus in the family Paramyxoviridae, is a highly contagious pathogen, affecting various domestic, and wild carnivores. Conventional methods are too cumbersome to be used for high-throughput screening of anti-CDV drugs. In this study, a recombinant CDV was rescued using reverse genetics for facilitating screening of anti-CDV drug in vitro. The recombinant CDV could stably express the NanoLuc® luciferase (NLuc), a novel enzyme that was smaller and “brighter” than others. The intensity of NLuc-catalyzed luminescence reaction indirectly reflected the anti-CDV effect of a certain drug, due to a positive correlation between NLuc expression and virus propagation in vitro. Based on such a characteristic feature, the recombinant CDV was used for anti-CDV assays on four drugs (ribavirin, moroxydine hydrochloride, 1-adamantylamine hydrochloride, and tea polyphenol) via analysis of luciferase activity, instead of via conventional methods. The result showed that out of these four drugs, only the ribavirin exhibited a detectable anti-CDV effect. The NLuc-tagged CDV would be a rapid tool for high-throughput screening of anti-CDV drugs.

Canine distemper virus associated proliferation of canine footpad keratinocytes in vitro

2005 ◽  
Vol 107 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
P. Engelhardt ◽  
M. Wyder ◽  
A. Zurbriggen ◽  
A. Gröne
Keyword(s):  
Canine Distemper Virus ◽  
Canine Distemper

In vitro efficacy of ribavirin against canine distemper virus

2008 ◽  
Vol 77 (2) ◽  
pp. 108-113 ◽  
Author(s):  
Gabriella Elia ◽  
Chiara Belloli ◽  
Francesco Cirone ◽  
Maria Stella Lucente ◽  
Marta Caruso ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Canine Distemper

scholarly journals Comparison between In Vitro Antiviral Effect of Mexican Propolis and Three Commercial Flavonoids against Canine Distemper Virus

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
María de Jesús González-Búrquez ◽  
Francisco Rodolfo González-Díaz ◽  
Carlos Gerardo García-Tovar ◽  
Liborio Carrillo-Miranda ◽  
Carlos Ignacio Soto-Zárate ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Cell Viability ◽  
Canine Distemper Virus ◽  
Antiviral Effect ◽  
Biological Properties ◽  
Cellular Viability ◽  
Canine Distemper ◽  
Viral Origin ◽  
Viral Expression

Propolis is a resin that honey bees (Apis mellifera) produce by mixing wax, exudates collected from tree shoots, pollen, and enzymes. It has been used for its biological properties against pathogenic microorganisms including those of viral origin. In the present study, we demonstrate the antiviral effect of Mexican propolis, as well as of the three commercial flavonoids (quercetin, naringenin, and pinocembrin) present in its composition, in cell cultures infected with Canine Distemper Virus. The treatments were carried out with propolis, flavonoids individually, and a mixture of the three flavonoids at three different times. Antiviral activity was evaluated by the inhibition of the relative expression of the virus nucleoprotein gene (Real-Time qPCR) and by the determination of cellular viability (MTT assay). Propolis applied before infection decreased viral expression (0.72 versus 1.0, 1.65, and 1.75 relative expressions) and correlated with increased cell viability (0.314 versus 0.215, 0.259, and 0.237 absorbance units (AU)). The administration of a flavonoid mixture containing the three commercial flavonoids before infection induces a slight decrease in viral expression (0.93 versus 1, 1.42, and 1.82 relative expressions); however, it does not improve cellular viability (0.255 versus 0.247, 0.282, and 0.245 AU). Quercetin administrated at the same time of infection decreases viral expression (0.90 versus 1.0, 3.25, and 1.02 relative expressions) and improves cellular viability (0.294 versus 0.240, 0.250, and 0.245 AU). Pinocembrin and naringenin individually did not show any antiviral activity at the administration times evaluated in this study. The present work is the first in vitro study of the effect of propolis in Canine Distemper Virus and demonstrated the antiviral activity of Mexican propolis, in addition to the synergy that exists between the three flavonoids on cell viability and the expression of the nucleoprotein virus gene.

scholarly journals Oxidative Stress in Canine Histiocytic Sarcoma Cells Induced by an Infection with Canine Distemper Virus Led to a Dysregulation of HIF-1α Downstream Pathway Resulting in a Reduced Expression of VEGF-B In Vitro

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 200 ◽  
Author(s):  
Federico Armando ◽  
Matteo Gambini ◽  
Attilio Corradi ◽  
Chiara Giudice ◽  
Vanessa Maria Pfankuche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Data Analysis ◽  
Microarray Data ◽  
Canine Distemper Virus ◽  
Histiocytic Sarcoma ◽  
Microarray Data Analysis ◽  
Canine Distemper ◽  
The Impact ◽  
Dh82 Cells

Histiocytic sarcomas represent malignant tumors which require new treatment strategies. Canine distemper virus (CDV) is a promising candidate due to its oncolytic features reported in a canine histiocytic sarcoma cell line (DH82 cells). Interestingly, the underlying mechanism might include a dysregulation of angiogenesis. Based on these findings, the aim of the present study was to investigate the impact of a persistent CDV-infection on oxidative stress mediated changes in the expression of hypoxia-inducible factor (HIF)-1α and its angiogenic downstream pathway in DH82 cells in vitro. Microarray data analysis, immunofluorescence for 8-hydroxyguanosine, superoxide dismutase 2 and catalase, and flow cytometry for oxidative burst displayed an increased oxidative stress in persistently CDV-infected DH82 cells (DH82Ond pi) compared to controls. The HIF-1α expression in DH82Ond pi increased, as demonstrated by Western blot, and showed an unexpected, often sub-membranous distribution, as shown by immunofluorescence and immunoelectron microscopy. Furthermore, microarray data analysis and immunofluorescence confirmed a reduced expression of VEGF-B in DH82Ond pi compared to controls. In summary, these results suggest a reduced activation of the HIF-1α angiogenic downstream pathway in DH82Ond pi cells in vitro, most likely due to an excessive, unusually localized, and non-functional expression of HIF-1α triggered by a CDV-induced increased oxidative stress.

Canine distemper virus in tissue culture

1958 ◽  
Vol 8 (4) ◽  
pp. 485-492 ◽  
Author(s):  
Gunnar Rockborn
Keyword(s):  
Tissue Culture ◽  
Canine Distemper Virus ◽  
Canine Distemper
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