3′-Terminal nucleotide sequence (N = 41) of satellite tobacco necrosis virus RNA

Virology ◽  
1979 ◽  
Vol 98 (1) ◽  
pp. 182-190 ◽  
Author(s):  
Jozef Merregaert ◽  
John Van Emmelo ◽  
Walter Fiers
Keyword(s):  
Nucleotide Sequence ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Terminal Nucleotide ◽  
Virus Rna ◽  
Terminal Nucleotide Sequence

scholarly journals The 3'-Terminal Nucleotide Sequence of Encephalomyocarditis Virus RNA

1978 ◽  
Vol 82 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Josef MERREGAERT ◽  
John EMMELO ◽  
Rene DEVOS ◽  
Alan PORTER ◽  
Peter FELLNER ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Encephalomyocarditis Virus ◽  
Terminal Nucleotide ◽  
Virus Rna ◽  
Terminal Nucleotide Sequence

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

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