Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide

Virology ◽  
1984 ◽  
Vol 135 (2) ◽  
pp. 316-330 ◽  
Author(s):  
Haruki Otsuka ◽  
Saul Kit
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Amino Acid Sequence ◽  
Thymidine Kinase ◽  
Predicted Amino Acid Sequence ◽  
Thymidine Kinase Gene ◽  
Kinase Gene

scholarly journals Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells.

1984 ◽  
Vol 4 (11) ◽  
pp. 2316-2320 ◽  
Author(s):  
H D Bradshaw ◽  
P L Deininger
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Thymidine Kinase ◽  
Mammalian Cells ◽  
Simian Virus 40 ◽  
Thymidine Kinase Gene ◽  
Coding Region ◽  
Base Pairs ◽  
Amino Acid Sequence Level ◽  
Kinase Gene

A cDNA containing the entire coding region of the human thymidine kinase gene has been molecularly cloned. The cDNA is under the control of a simian virus 40 promoter and is expressible in mammalian cells. The complete nucleotide sequence of the human thymidine kinase cDNA has been determined. The cDNA is 1,421 base pairs in length and has a large open reading frame of 702 base pairs capable of specifying a protein with a molecular weight of 25,504. Genomic Southern blotting experiments show that sequences homologous to the human thymidine kinase cDNA are conserved among many vertebrates, including prosimians (lemur), tree shrews, rats, mice, and chickens. Direct comparison of the nucleotide sequences of the human thymidine kinase cDNA and the chicken thymidine kinase gene reveals ca. 70% overall homology. This homology is extended further at the amino acid sequence level, with greater than 74% amino acid residues matched between the human and chicken thymidine kinase proteins.

Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells

1984 ◽  
Vol 4 (11) ◽  
pp. 2316-2320 ◽  
Author(s):  
H D Bradshaw ◽  
P L Deininger
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Thymidine Kinase ◽  
Mammalian Cells ◽  
Simian Virus 40 ◽  
Thymidine Kinase Gene ◽  
Coding Region ◽  
Base Pairs ◽  
Amino Acid Sequence Level ◽  
Kinase Gene

A cDNA containing the entire coding region of the human thymidine kinase gene has been molecularly cloned. The cDNA is under the control of a simian virus 40 promoter and is expressible in mammalian cells. The complete nucleotide sequence of the human thymidine kinase cDNA has been determined. The cDNA is 1,421 base pairs in length and has a large open reading frame of 702 base pairs capable of specifying a protein with a molecular weight of 25,504. Genomic Southern blotting experiments show that sequences homologous to the human thymidine kinase cDNA are conserved among many vertebrates, including prosimians (lemur), tree shrews, rats, mice, and chickens. Direct comparison of the nucleotide sequences of the human thymidine kinase cDNA and the chicken thymidine kinase gene reveals ca. 70% overall homology. This homology is extended further at the amino acid sequence level, with greater than 74% amino acid residues matched between the human and chicken thymidine kinase proteins.

scholarly journals Molecular Characterization of an Avian Astrovirus

2000 ◽  
Vol 74 (13) ◽  
pp. 6173-6177 ◽  
Author(s):  
Matthew D. Koci ◽  
Bruce S. Seal ◽  
Stacey Schultz-Cherry
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Amino Acid Sequence ◽  
Animal Species ◽  
Genomic Organization ◽  
Predicted Amino Acid Sequence ◽  
Enteric Disease ◽  
Nucleotide Level ◽  
Human Astroviruses

ABSTRACT Astroviruses are known to cause enteric disease in several animal species, including turkeys. However, only human astroviruses have been well characterized at the nucleotide level. Herein we report the nucleotide sequence, genomic organization, and predicted amino acid sequence of a turkey astrovirus isolated from poults with an emerging enteric disease.

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