Biological evaluation of glycoproteins mapping to two distinct mRNAs within the BamHI fragment 7 of pseudorabies virus: Expression of the coding regions by vaccinia virus

Virology ◽  
1989 ◽  
Vol 171 (2) ◽  
pp. 365-376 ◽  
Author(s):  
Thomas A. Kost ◽  
Elaine V. Jones ◽  
Kaye M. Smith ◽  
A. Paul Reed ◽  
Albert L. Brown ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Pseudorabies Virus ◽  
Biological Evaluation ◽  
Bamhi Fragment ◽  
Coding Regions ◽  
Virus Expression

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

scholarly journals SYNTHESIS AND BIOLOGICAL EVALUATION OF 1H-PYRAZOLO [3,4-b] PYRIDINE-5 CARBOXYLIC ACIDS AGAINST VACCINIA VIRUS

2002 ◽  
Vol 8 (5) ◽  
Author(s):  
Alexandre R. Azevedo ◽  
Vitor F. Ferreira ◽  
Heloisa de Mello ◽  
Luiz R. Leão-Ferreirab ◽  
Alfredo V. Jabor ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Carboxylic Acids ◽  
Biological Evaluation ◽  
Synthesis And Biological Evaluation

Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response

Vaccine ◽  
1994 ◽  
Vol 12 (5) ◽  
pp. 465-471 ◽  
Author(s):  
Travis C. McGuire ◽  
Edward B. Stephens ◽  
Guy H. Palmer ◽  
Terry F. McElwain ◽  
Carol A. Lichtensteiger ◽  
...  
Keyword(s):  
Antibody Response ◽  
Vaccinia Virus ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Recombinant Vaccinia Virus ◽  
Gpi Anchor ◽  
Recombinant Vaccinia ◽  
Anchor Sequence ◽  
Virus Expression

Vaccinia Virus Expression Vectors

1987 ◽  
Vol 5 (1) ◽  
pp. 305-324 ◽  
Author(s):  
B Moss ◽  
C Flexner
Keyword(s):  
Vaccinia Virus ◽  
Expression Vectors ◽  
Virus Expression

Study of the murine Hox-2.3 protein using a vaccinia virus expression system

1989 ◽  
Vol 27 ◽  
pp. 186
Author(s):  
Ron de Jong ◽  
Lia de Laaf ◽  
Harry Vennema ◽  
Willy Spaan ◽  
Jacqueline Deschamps ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Virus Expression

Functional Characterization of Glycosylation-Deficient Human P-Glycoprotein Using A Vaccinia Virus Expression System

2000 ◽  
Vol 173 (3) ◽  
pp. 203-214 ◽  
Author(s):  
J.J. Gribar ◽  
M. Ramachandra ◽  
C.A. Hrycyna ◽  
S. Dey ◽  
S.V. Ambudkar
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Functional Characterization ◽  
P Glycoprotein ◽  
Virus Expression
Sign in / Sign up

Export Citation Format

Share Document