Vaccinia Virus Expression Vectors

1987 ◽  
Vol 5 (1) ◽  
pp. 305-324 ◽  
Author(s):  
B Moss ◽  
C Flexner
Keyword(s):  
Vaccinia Virus ◽  
Expression Vectors ◽  
Virus Expression

Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype

Nature ◽  
1985 ◽  
Vol 317 (6040) ◽  
pp. 813-815 ◽  
Author(s):  
R. M. L. Buller ◽  
G. L. Smith ◽  
K. Cremer ◽  
A. L. Notkins ◽  
Bernard Moss
Keyword(s):  
Vaccinia Virus ◽  
Thymidine Kinase ◽  
Recombinant Vaccinia Virus ◽  
Expression Vectors ◽  
Recombinant Vaccinia ◽  
Virus Expression ◽  
Negative Phenotype

Vaccinia Virus Expression Vectors

1989 ◽  
Vol 569 (1 Biomedical Sc) ◽  
pp. 86-103 ◽  
Author(s):  
BERNARD MOSS ◽  
CHARLES FLEXNER
Keyword(s):  
Vaccinia Virus ◽  
Expression Vectors ◽  
Virus Expression

Vaccinia Virus Expression Vectors

1986 ◽  
Vol 67 (10) ◽  
pp. 2067-2082 ◽  
Author(s):  
M. Mackett ◽  
G. L. Smith
Keyword(s):  
Vaccinia Virus ◽  
Expression Vectors ◽  
Virus Expression

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response

Vaccine ◽  
1994 ◽  
Vol 12 (5) ◽  
pp. 465-471 ◽  
Author(s):  
Travis C. McGuire ◽  
Edward B. Stephens ◽  
Guy H. Palmer ◽  
Terry F. McElwain ◽  
Carol A. Lichtensteiger ◽  
...  
Keyword(s):  
Antibody Response ◽  
Vaccinia Virus ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Recombinant Vaccinia Virus ◽  
Gpi Anchor ◽  
Recombinant Vaccinia ◽  
Anchor Sequence ◽  
Virus Expression
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