Control and expression of 3′ open reading frames in clover yellow mosaic virus

Virology ◽  
1990 ◽  
Vol 179 (2) ◽  
pp. 576-584 ◽  
Author(s):  
K.Andrew White ◽  
George A. Mackie
Keyword(s):  
Mosaic Virus ◽  
Open Reading Frames ◽  
Yellow Mosaic Virus ◽  
Clover Yellow Mosaic Virus ◽  
Reading Frames

scholarly journals Cloning and sequence analysis of an infectious clone of Citrus yellow mosaic virus that can infect sweet orange via Agrobacterium-mediated inoculation

2001 ◽  
Vol 82 (10) ◽  
pp. 2549-2558 ◽  
Author(s):  
Qi Huang ◽  
John S. Hartung
Keyword(s):  
Mosaic Virus ◽  
Sweet Orange ◽  
Infectious Clone ◽  
Mosaic Disease ◽  
Open Reading Frames ◽  
Yellow Mosaic Virus ◽  
Virus Movement ◽  
The Family ◽  
Cacao Swollen Shoot Virus ◽  
Reading Frames

Citrus yellow mosaic virus (CYMV), a member of the family Caulimoviridae, genus Badnavirus, causes citrus mosaic disease, a disease that occurs commonly in India. The CYMV genome has been cloned and its complete nucleotide sequence determined. Its DNA genome is 7559 bp in length and contains six putative open reading frames (ORFs), all on the plus-strand of the genome and each capable of encoding proteins with a molecular mass of greater than 10 kDa. ORF 3, the largest ORF, encodes a putative polyprotein for functions involved in virus movement, assembly and replication. The other ORFs encode proteins whose exact functions are not completely understood. The genome also contains a plant tRNAmet-binding site, which may serve as a primer for minus-strand DNA synthesis, in its intergenic region. Phylogenetic analysis of the badnaviruses revealed that CYMV is most closely related to Cacao swollen shoot virus. It was demonstrated that a construct containing 1·4 copies of the cloned CYMV genome could infect sweet orange via Agrobacterium-mediated inoculation.

scholarly journals Evolutionary History of Cucumber Mosaic Virus Deduced by Phylogenetic Analyses

2002 ◽  
Vol 76 (7) ◽  
pp. 3382-3387 ◽  
Author(s):  
Marilyn J. Roossinck
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Isolate ◽  
Phylogenetic Analyses ◽  
Open Reading Frames ◽  
Broad Host Range ◽  
Nontranslated Region ◽  
History Of ◽  
Evolutionary Success ◽  
Reading Frames

ABSTRACT Cucumber mosaic virus (CMV) is an RNA plant virus with a tripartite genome and an extremely broad host range. Previous evolutionary analyses with the coat protein (CP) and 5′ nontranslated region (NTR) of RNA 3 suggested subdivision of the virus into three groups, subgroups IA, IB, and II. In this study 15 strains of CMV whose nucleotide sequences have been determined were used for a complete phylogenetic analysis of the virus. The trees estimated for open reading frames (ORFs) located on the different RNAs were not congruent and did not completely support the subgrouping indicated by the CP ORF, indicating that different RNAs had independent evolutionary histories. This is consistent with a reassortment mechanism playing an important role in the evolution of the virus. The evolutionary trees of the 1a and 3a ORFs were more compact and displayed more branching than did those of the 2a and CP ORFs. This may reflect more rigid host-interactive constraints exerted on the 1a and 3a ORFs. In addition, analysis of the 3′ NTR that is conserved among all RNAs indicated that evolutionary constraints on this region are specific to the RNA component rather than the virus isolate. This indicates that functions other than replication are encoded in the 3′ NTR. Reassortment may have led to the genetic diversity found among CMV strains and contributed to its enormous evolutionary success.

Purification of Clover Yellow Mosaic Virus

Nature ◽  
1962 ◽  
Vol 194 (4826) ◽  
pp. 408-409 ◽  
Author(s):  
H. AGRAWAL ◽  
M. CHESSIN ◽  
L. BOS
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Clover Yellow Mosaic Virus

scholarly journals Viruses of White Clover in Pastures of Pennsylvania, New York, and Vermont

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 817-820 ◽  
Author(s):  
Robert T. Sherwood
Keyword(s):  
New York ◽  
Mosaic Virus ◽  
White Clover ◽  
Significant Variation ◽  
Alfalfa Mosaic Virus ◽  
Red Clover ◽  
Yellow Mosaic Virus ◽  
Peanut Stunt Virus ◽  
Clover Yellow Mosaic Virus ◽  
White Clover Mosaic Virus

Incidence of six viruses was tested in white clover from 28 rotationally grazed pastures of Pennsylvania (PA), New York (NY), and Vermont (VT). Each of 17 PA pastures was sampled fall 1994, spring 1995, fall 1995, and spring 1996, and 10 pastures were sampled fall 1996. Each of five NY and six VT pastures was sampled spring and fall 1995 and 1996. Enzyme-linked immunosorbent assays (ELISA) were conducted for red clover vein mosaic virus (RCVMV), white clover mosaic virus (WCMV), alfalfa mosaic virus (AlMV), peanut stunt virus (PSV), clover yellow mosaic virus (CYMV), and the potyvirus group (POTY). RCVMV, WCMV, AlMV, and POTY were detected in 28, 28, 27, and 25 of the 28 pastures and in 67, 32, 30, and 7% of the 3,065 samples tested, respectively. PSV occurred at low to moderate levels in 11 PA pastures. PSV was rare in NY and was not detected in VT. CYMV was never found. Incidence of each virus varied significantly among pastures. For any given virus, there was not a significant variation in incidence among sampling dates within the NY-VT samples. RCVMV, WCMV, and POTY varied among dates within PA.

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