Energy loss spectroscopic imaging of rat ameloblasts

1985 ◽  
Vol 16 ◽  
pp. 175
Author(s):  
K. Siangchaew ◽  
J. Bentley ◽  
M. Libera

Energy-filtered electron-spectroscopic TEM imaging provides a new way to study the microstructure of polymers without heavy-element stains. Since spectroscopic imaging exploits the signal generated directly by the electron-specimen interaction, it can produce richer and higher resolution data than possible with most staining methods. There are basically two ways to collect filtered images (fig. 1). Spectrum imaging uses a focused probe that is digitally rastered across a specimen with an entire energy-loss spectrum collected at each x-y pixel to produce a 3-D data set. Alternatively, filtering schemes such as the Zeiss Omega filter and the Gatan Imaging Filter (GIF) acquire individual 2-D images with electrons of a defined range of energy loss (δE) that typically is 5-20 eV.


Planta ◽  
1999 ◽  
Vol 208 (3) ◽  
pp. 438-443 ◽  
Author(s):  
I. Max Huxham ◽  
Michael C. Jarvis ◽  
Lynette Shakespeare ◽  
Colin J. Dover ◽  
David Johnson ◽  
...  

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