Regulation of insecticide resistance-related cytochrome P-450 expression in Drosophila melanogaster

1988 ◽  
Vol 30 (3) ◽  
pp. 214-227 ◽  
Author(s):  
Larry C. Waters ◽  
Carroll E. Nix
1990 ◽  
Vol 265 (1) ◽  
pp. 213-217 ◽  
Author(s):  
S S Sundseth ◽  
C E Nix ◽  
L C Waters

Significant purification of the ubiquitous cytochrome P-450-A and the strain-specific P-450-B from Drosophila melanogaster has been achieved by sequential chromatography on octylamino-agarose, DEAE-cellulose and hydroxyapatite. Preparations of P-450-A (specific contents of 7-9 nmol/mg) were homogeneous as determined by SDS/polyacrylamide-gel electrophoresis (PAGE) analysis. Preparations enriched for P-450-B (specific contents of 4-7 nmol/mg) contained significant amounts of P-450-A but were essentially free of other proteins as judged by SDS/PAGE. Partial reconstitution of 7-ethoxycoumarin de-ethylase activity was achieved using rabbit NADPH: cytochrome P450 reductase and purified preparations containing P450-B.


2020 ◽  
Vol 29 (14) ◽  
pp. 2661-2675 ◽  
Author(s):  
Rafael A. Homem ◽  
Bliss Buttery ◽  
Ewan Richardson ◽  
Yao Tan ◽  
Linda M. Field ◽  
...  

2012 ◽  
Vol 42 (12) ◽  
pp. 918-924 ◽  
Author(s):  
Phillip J. Daborn ◽  
Christopher Lumb ◽  
Thomas W.R. Harrop ◽  
Alex Blasetti ◽  
Shivani Pasricha ◽  
...  

2013 ◽  
Vol 60 ◽  
pp. 355-359 ◽  
Author(s):  
Predrag Kalajdzic ◽  
Maria Markaki ◽  
Stefan Oehler ◽  
Charalambos Savakis

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