Histone gene transcripts in the cleavage and mesenchyme blastula embryo of the sea urchin, S. purpuratus

Cell ◽  
1978 ◽  
Vol 14 (2) ◽  
pp. 313-326 ◽  
Author(s):  
Nancy Stover Kunkel ◽  
Eric S. Weinberg
Keyword(s):  
Sea Urchin ◽  
Histone Gene ◽  
Gene Transcripts

Size of histone gene transcripts in different embryonic stages of the sea urchin, Strongylocentrotus purpuratus

Biochemistry ◽  
1978 ◽  
Vol 17 (13) ◽  
pp. 2591-2598 ◽  
Author(s):  
Nancy S. Kunkel ◽  
Kari Hemminki ◽  
Eric S. Weinberg
Keyword(s):  
Sea Urchin ◽  
Strongylocentrotus Purpuratus ◽  
Histone Gene ◽  
Gene Transcripts

scholarly journals Stimulation of sea urchin H2B histone gene transcription by a chromatin-associated protein fraction depends on gene sequences downstream of the transcription start site.

1985 ◽  
Vol 5 (10) ◽  
pp. 2764-2769 ◽  
Author(s):  
J Mous ◽  
H Stunnenberg ◽  
O Georgiev ◽  
M L Birnstiel
Keyword(s):  
Sea Urchin ◽  
Protein Fraction ◽  
Transcription Initiation ◽  
Initiation Site ◽  
Histone Gene ◽  
Xenopus Laevis Oocyte ◽  
Chromosomal Protein ◽  
Base Pairs ◽  
Sequence Element ◽  
Associated Proteins

We isolated a chromosomal protein fraction derived from chromatin of sea urchin embryos which specifically stimulated the expression of the histone H2B gene by a factor of 5- to 10-fold when the complete sea urchin histone gene repeat h22 was injected in Xenopus laevis oocyte nuclei. Gene manipulation experiments revealed the existence of two different target sites in the H2B gene which appear to mediate the response to injection of the stimulatory sea urchin chromatin-associated proteins; both are located downstream of the transcription initiation site. The first sequence element which is shown to be implicated is within, or at least includes, the H2B 5' untranslated leader sequence between nucleotides 11 and 76. The second element resides within an H2B DNA segment located near the 3' end of the gene, extending from 90 base pairs upstream of the mRNA 3' terminus to 140 base pairs in the spacer sequences downstream.

scholarly journals Enhancer blocking activity located near the 3' end of the sea urchin early H2A histone gene

1997 ◽  
Vol 94 (6) ◽  
pp. 2272-2277 ◽  
Author(s):  
F. Palla ◽  
R. Melfi ◽  
L. Anello ◽  
M. Di Bernardo ◽  
G. Spinelli
Keyword(s):  
Sea Urchin ◽  
Histone Gene ◽  
Blocking Activity ◽  
Enhancer Blocking

scholarly journals An unusual evolutionary behaviour of a sea urchin histone gene cluster

1982 ◽  
Vol 1 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Meinrad Busslinger ◽  
Sandro Rusconi ◽  
Max L. Birnstiel
Keyword(s):  
Gene Cluster ◽  
Sea Urchin ◽  
Histone Gene

Localization of histone gene transcripts in newt lampbrush chromosomes by in situ hybridization

1977 ◽  
Vol 27 (1) ◽  
pp. 57-79
Author(s):  
R.W. Old ◽  
G.H. Callan ◽  
K.W. Gross
Keyword(s):  
Dna Sequences ◽  
Histone Gene ◽  
Lampbrush Chromosomes ◽  
Rna Sequences ◽  
Psammechinus Miliaris ◽  
Lateral Loop ◽  
Gene Transcripts ◽  
Label Distribution ◽  
Chromosome I

Denatured 3H-labelled DNAs containing histone gene sequences originating from the echinoderms Psammechinus miliaris, Echimus esculentus and Strongylocentrotus purpuratus have been in situ hybridized to RNA transcripts on newt lampbrush chromosomes. Autoradiographs of the hybridized lampbrush preparations show labelling restricted to four or fewer lateral loop pairs all lying within the heteromorphic regions of chromosome I, also one or two loop pairs on chromosome VI, one loop pair on chromosome X and one loop pair on chromosome XI. For oocytes from a single newt, coincident label distribution is found with DNA's of diverse echinoderm origin; however different newts show some specific individual diversity in label distribution, including heterozygosity in the case of loops on bivalents VI and X. The more conspicuously labelled loops, particularly those on chromosome I, show a pattern of labelling which is explicable if the newt histone DNA sequences are confined to short intercepts of lateral loop axis. Transcription is initiated prior to the histone DNA sequences, proceeds through the histone DNA sequences, and beyond, and the histone RNA sequences are cut from the transcripts before the termination of transcription.

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