The E. coli cell surface specifically prevents the initiation of DNA replication at oriC on heminethylated DNA templates

Cell ◽  
1990 ◽  
Vol 63 (5) ◽  
pp. 1053-1060 ◽  
Author(s):  
Ahmed Landoulsi ◽  
Abderrahim Malki ◽  
Reneé Kern ◽  
Masamichi Kohlyama ◽  
Patrick Hughes
Keyword(s):  
Dna Replication ◽  
Cell Surface ◽  
Coli Cell ◽  
Dna Templates ◽  
E Coli ◽  
Initiation Of Dna Replication

scholarly journals Selective Inhibition by Pantoyl Lactone and Butyl Alcohol of the Initiation of DNA Replication in E. coli

1973 ◽  
Vol 37 (6) ◽  
pp. 1317-1320
Author(s):  
Yoshihiro YOSHIYAMA ◽  
Kazuo NAGAI ◽  
Hideaki SOME ◽  
Gakuzo TAMURA
Keyword(s):  
Dna Replication ◽  
Selective Inhibition ◽  
Butyl Alcohol ◽  
E Coli ◽  
Initiation Of Dna Replication

scholarly journals Molecular cloning of mtp70, a mitochondrial member of the hsp70 family.

1989 ◽  
Vol 9 (11) ◽  
pp. 5163-5168 ◽  
Author(s):  
D M Engman ◽  
L V Kirchhoff ◽  
J E Donelson
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Protozoan Parasite ◽  
Specific Region ◽  
Kinetoplast Dna ◽  
Intracellular Location ◽  
E Coli ◽  
Hsp70 Family ◽  
Mitochondrial Dna Replication ◽  
Initiation Of Dna Replication

We have isolated a gene from the protozoan parasite Trypanosoma cruzi that encodes a previously unidentified member of the 70-kilodalton heat shock protein (hsp70) family. Among all the eucaryotic hsp70 proteins described to date, this trypanosome protein, mtp70, is uniquely related in sequence and structure to the hsp70 of Escherichia coli, DnaK, which functions in the initiation of DNA replication. This relationship to DnaK is especially relevant in view of the intracellular location of the protein. Within the trypanosome, mtp70 is located in the mitochondrion, where it associates with kinetoplast DNA (kDNA), the unusual mitochondrial DNA that distinguishes this order of protozoa. Moreover, mtp70 is located in the specific region of the kinetoplast in which kDNA replication occurs. In view of the known functions of DnaK, the localization of mtp70 to the site of kDNA replication suggests that mtp70 may participate in eucaryotic mitochondrial DNA replication in a manner analogous to that of DnaK in E. coli.

Molecular cloning of mtp70, a mitochondrial member of the hsp70 family

1989 ◽  
Vol 9 (11) ◽  
pp. 5163-5168
Author(s):  
D M Engman ◽  
L V Kirchhoff ◽  
J E Donelson
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Protozoan Parasite ◽  
Specific Region ◽  
Kinetoplast Dna ◽  
Intracellular Location ◽  
E Coli ◽  
Hsp70 Family ◽  
Mitochondrial Dna Replication ◽  
Initiation Of Dna Replication

We have isolated a gene from the protozoan parasite Trypanosoma cruzi that encodes a previously unidentified member of the 70-kilodalton heat shock protein (hsp70) family. Among all the eucaryotic hsp70 proteins described to date, this trypanosome protein, mtp70, is uniquely related in sequence and structure to the hsp70 of Escherichia coli, DnaK, which functions in the initiation of DNA replication. This relationship to DnaK is especially relevant in view of the intracellular location of the protein. Within the trypanosome, mtp70 is located in the mitochondrion, where it associates with kinetoplast DNA (kDNA), the unusual mitochondrial DNA that distinguishes this order of protozoa. Moreover, mtp70 is located in the specific region of the kinetoplast in which kDNA replication occurs. In view of the known functions of DnaK, the localization of mtp70 to the site of kDNA replication suggests that mtp70 may participate in eucaryotic mitochondrial DNA replication in a manner analogous to that of DnaK in E. coli.

The E. coli cell surface: Isolation of λ transducing phages carrying the tolPAB cluster

1973 ◽  
Vol 121 (4) ◽  
pp. 325-335 ◽  
Author(s):  
Alan Bernstein ◽  
Barry Rolfe ◽  
Kazukiyo Onodera
Keyword(s):  
Cell Surface ◽  
Coli Cell ◽  
E Coli

scholarly journals Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hamidreza Fasehee ◽  
Amin Rostami ◽  
Fatemeh Ramezani ◽  
Gholamreza Ahmadian
Keyword(s):  
Cell Surface ◽  
Recombinant Proteins ◽  
Coli Cell ◽  
Purification Method ◽  
E Coli ◽  
One Step
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