The low affinity binding sites of the vitamin D-dependent calcium-binding protein and their functional role in calcium transport

Cell Calcium ◽  
1981 ◽  
Vol 2 (1) ◽  
pp. 77-88 ◽  
Author(s):  
V.K. Bauman ◽  
Y.Y. Galvanovsky
Keyword(s):  
Vitamin D ◽  
Binding Sites ◽  
Calcium Binding ◽  
Calcium Transport ◽  
Functional Role ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Affinity Binding

scholarly journals Immunoperoxidase localization of vitamin D dependent calcium binding protein.

1978 ◽  
Vol 26 (8) ◽  
pp. 628-634 ◽  
Author(s):  
R L Morrissey ◽  
D T Zolock ◽  
T J Bucci ◽  
D D Bikle
Keyword(s):  
Vitamin D ◽  
Calcium Binding ◽  
Functional Role ◽  
Binding Protein ◽  
Goblet Cells ◽  
Calcium Binding Protein ◽  
Intracellular Accumulation ◽  
Absorptive Cells ◽  
Antibody Method

Calcium binding protein (CaBP) was localized by the indirect peroxidase-labeled antibody method in chick duodenum 72 hr after administering 32.5 nmol of cholecalciferol to vitamin D-deficient chicks. CaBP was observed in cytoplasm and nuclei of absorptive cells but was absent from goblet cells. Our results are consistent with the suggested functional role for CaBP in the prevention of intracellular accumulation of calcium by preventing mitochondrial accumulation of calcium, enhancing removal of calcium from absorptive cells, and/or preventing the "leaking" of calcium into cells through the lateral borders. They are not consistent with an extracellular functional role for CaBP.

Intestinal vitamin D-induced calcium-binding protein: time-course of immunocytological localization following 1,25-dihydroxyvitamin D3.

1983 ◽  
Vol 31 (3) ◽  
pp. 426-432 ◽  
Author(s):  
A N Taylor
Keyword(s):  
Vitamin D ◽  
Calcium Binding ◽  
Calcium Transport ◽  
Time Course ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
Migration Rates ◽  
1,25 Dihydroxyvitamin D3 ◽  
Free Cells

The vitamin D-induced calcium-binding protein (CaBP) was localized in histological sections of chick duodenum using the peroxidase-antiperoxidase immunocytochemical technique. The time-course of appearance of CaBP in rachitic chicks was investigated from 0 to 120 hr after stimulation by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). CaBP was not routinely detected at 0 hr after 1,25(OH)2D3 administration. CaBP was first noted in some, but not all, of the samples taken 2 hr following 1,25(OH)2D3 and was detected in all 2 1/2 hr samples. The number of CaBP-containing absorptive cells and the apparent CaBP concentration both increased to a maximum at about 16-24 hr. At later times, as CaBP free cells migrated up the villi, the CaBP-containing cells decreased in number, but even at 120 hr post 1,25(OH)2D3 dose there were significant numbers of CaBP-containing cells present. The relationships between time-course of CaBP location on intestinal villi, enterocyte migration rates, and the time-course of 1,25(OH)2D3 stimulated intestinal calcium transport are discussed.

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