Isolation and amino acid sequence of calcitonin gene related peptide from porcine spinal cord

Neuropeptides ◽  
1987 ◽  
Vol 9 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Sadao Kimura ◽  
Yoshiki Sugita ◽  
Ichiro Kanazawa ◽  
Akira Saito ◽  
Katsutoshi Golo
Clinics ◽  
2012 ◽  
Vol 67 (9) ◽  
pp. 1087-1091 ◽  
Author(s):  
PS do Nascimento ◽  
GA Lovatel ◽  
J Ilha ◽  
LL Xavier ◽  
BD Schaan ◽  
...  

1988 ◽  
Vol 50 (2) ◽  
pp. 480-485 ◽  
Author(s):  
Osamu Hiroshima ◽  
Yoshihisa Sano ◽  
Teruaki Yuzuriha ◽  
Chiyuki Yamato ◽  
Akira Saito ◽  
...  

1989 ◽  
Vol 3 (3) ◽  
pp. 247-252 ◽  
Author(s):  
S. J. Wimalawansa ◽  
H. R. Morris ◽  
I. Maclntyre

ABSTRACT The presence of both α- and β-calcitonin gene-related peptide (CGRP) was demonstrated by oxidation and by CNBr cleavage in extracts of plasma, cerebrospinal fluid and spinal cord in man. This was achieved by the use of both CNBr cleavage and oxidation of the methionine residue present in the human β-CGRP molecule. This study demonstrates that around 50% of CGRP immunoreactivity in plasma, cerebrospinal fluid and spinal cord is not α-CGRP, but corresponds to β-CGRP-like activity. Furthermore, experiments with CNBr also suggest the presence of another methionine-containing CGRP-like peptide in all three extracts.


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